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Expression data from human primary keratinocytes

ABSTRACT: The genetic expression profile of a Wnt signal agonist, BIO, was evaluated in human primary keratinocytes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE40233 | GEO | 2012/10/01

SECONDARY ACCESSION(S): PRJNA173246

REPOSITORIES: GEO

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Expression data from human primary keratinocytes

Project description:The genetic expression profile of a Wnt signal agonist, BIO, was evaluated in human primary keratinocytes. Accelerating scaling up of primary keratinocytes benefits skin autografts for severely burned patients. Wnt signal, a conserved pathway controlling cell cycle and morphogenesis of embryo, has been postulated to promote the cell proliferation and tumorigenesis in adult. Here, the effects of Wnt signal on the growth of interfollicular keratinocytes were investigated. We demonstrated that recombinant Wnt3a significantly promoted the primary keratinocyte growth at a low cell density. A well-characterized GSK-3beta inhibitor, BIO, activated the Wnt signals and also enhanced the colony formation of keratinocytes dose-dependently. Gene expression profile of the BIO-treated keratinocytes revealed the linkage of the BIO with the cell mitosis and indicated that epithelial cell adhesion molecule (EpCAM), a Wnt target gene, was upregulated. Comparing to the EpCAM- keratinocytes, the EpCAM+ cells showed higher proliferation rate and efficacy of the colony formation. Especially, inhibiting the EpCAM expression by shRNA attenuated the proliferation effect of BIO and the growth advantage of the EpCAM+ keratinocytes. These evidences emphasize the positive role of canonical Wnt and EpCAM on the regulation of cell growth and self-renewal for human keratinocytes.

2012-10-01 | E-GEOD-40233 | biostudies-arrayexpress

Expression data from BIO-treated human epidermal keratinocytes

2011-02-24 | GSE27509 | GEO

Expression data from BIO-treated human epidermal keratinocytes

Project description:Human keratinocytes isolated from foreskin were cultured and treated with a GSK-3beta inhibitor, BIO. The effect of BIO was evaluated by the cell growth, clony formation and differentiating markers. We used microarrays to detail the global program of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process. Human primary keratinocytes were subjected for RNA extraction. The total RNAs were hybridized on Affymetrix microarrays. The cultured cells exhibit a homogous cell morphology at passage 1 to passage 5. The cells were treated with BIO at passage 3 or 4.

2011-02-24 | E-GEOD-27509 | biostudies-arrayexpress

Expression profile of primary Keratinocytes and E13.5 MEFs

Project description:Expression profile of primary Keratinocytes and E13.5 MEFs

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Expression data from Sos1 and Sos2-depleted (individally or combined) mouse primary keratinocytes

Project description:Sos proteins are involved in the maintaining of skin homeostasis and participate in the initiation/progression of chemically-induced skin cancer. However, the precise role of these proteins in specific skin cellular compartments remains undetermined. Here, we examined the role of Sos1 and Sos2 in the overall transcriptional profile of mouse primary keratinocytes in order to unveil the specific functions of these Ras activators in keratinocyte population. We used microarrays to detail the impact of Sos1/2 depletion (individual or combined) in the transcriptional signature of mouse primary keratinocytes

2021-02-03 | GSE166020 | GEO

Expression data from LINC00958-depleted primary human keratinocytes

Project description:Long non-coding RNAs (lncRNAs) have emerged as important regulators of biological processes and diseases, the function of the majority of epidermal lncRNAs in skin homeostasis and their potential role in psoriasis has remained unknown. We report the identification of lncRNAs with altered expression in psoriasis keratinocytes and identify LINC00958 is overexpressed in psoriasis epidermis. We used microarrays to detail the global programme of gene expression upon LINC00958 depletion in keratinocytes

2022-09-30 | GSE205499 | GEO

Expression data from LINC00958-depleted primary human keratinocytes

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Gene expression analysis between primary keratinocytes and primary dermal fibroblasts obtained from the same skin sample

Project description:Gene expression profile was obtained from primary keratinocytes and primary dermal fibroblasts obtained from the same skin sample

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Expression data from autophagy-deficient keratinocytes and normal keratinocytes

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Expression data from primary human keratinocytes upon control or HERC6 knockdown using siRNA and stimulated in vitro with or without cGAMP or Poly(I:C)

Project description:HERC6 is an Interferon stimulated gene and expressed by epidermal keratinocytes, yet its specific role in the skin remains unclear. In this investigation, we employed bulk RNA-sequencing to explore the broader impact of HERC6 in response to sensing of cytosolic DNA mimic, cGAMP or RNA agonist, TLR3. We observed an elevated number of differentially expressed genes in keratinocytes following HERC6 knockdown, and among them, several were associated with the Interferon signaling pathway, such as OAS1, IFIT3, and STAT2, showing increased expression in HERC6 knockdown keratinocytes when stimulated with cGAMP. Additionally, our findings revealed a dysregulation in the HIPPO pathway-related genes, particularly LATS1/2, in HERC6 knockdown keratinocytes. Utilizing bulk RNA-sequencing, we identified enriched Gene Ontology terms associated with the Interferon pathway and cytosolic sensing of foreign DNA in HERC6 knockdown keratinocytes, suggesting HERC6 as a negative regulator of Interferon signaling upon DNA sensing but not RNA. This data was instrumental in elucidating the regulatory functions of HERC6 in keratinocytes during the sensing of exogenous DNA or RNA mimics.

2024-02-14 | GSE253221 | GEO

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