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Generation of induced oligodendrocyte progenitor cells (OPCs) from human somatic cells by OCT4

ABSTRACT: Generation of induced oligodendrocyte progenitor cells (OPCs) from human somatic cells by OCT4

PROVIDER: PRJNA540652 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8996030_1.fastq.gz	Fastqsanger.gz
	SRR8996030_2.fastq.gz	Fastqsanger.gz
	SRR8996040_1.fastq.gz	Fastqsanger.gz
	SRR8996040_2.fastq.gz	Fastqsanger.gz
	SRR8996031.fastq.gz	Fastqsanger.gz

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Generation of induced oligodendrocyte progenitor cells (OPCs) from human somatic cells by OCT4

Project description:The goal of this study is to identify the role of OCT4 in combination with small molecules as the critical cue governing the reprogramming of human somatic cells into OPCs using ChIP-seq

2019-05-02 | GSE130565 | GEO

Transcriptome analysis of freshly isolated young and aged rat oligodendrocyte progenitor cells (OPCs)

Project description:The age-related failure to regenerate lost oligodendrocytes from oligodendrocyte progenitor cells (OPCs) is associated with irreversible neurodegeneration in multiple sclerosis. Consequently, regenerative therapies for chronic demyelinating diseases remain a long sought after but elusive goal. Here we show that adult OPCs lose their inherent differentiation potential with increasing age. Moreover, aged OPCs do not remain responsive to pro-differentiation signals, posing significant constraints on the effectiveness of remyelination therapies based on the enhancement of OPC differentiation. We show that this reduced regenerative capacity is associated with hallmarks of cellular ageing, including reduced metabolic function and increased DNA damage. We find that fasting or treatment with metformin can reverse these changes and restore the regenerative capacity of aged OPCs, improving remyelination in aged animals following white matter injury. Importantly, aged OPCs treated with metformin regain their responsiveness to pro-differentiation factors, suggesting a potential for synergistic effects of rejuvenation and pro-differentiation therapies. Our findings have direct implications for understanding oligodendrocyte regeneration failure with ageing and how it might be reversed therapeutically.

2019-11-01 | GSE134765 | GEO

Gene expression analysis after GPR17 silencing in oligodendrocyte precursor cells (OPCs)

Project description:GPR17 silencing in OPCs accelerates their differentiation into fully mature oligodendrocytes. We performed a whole microarray profiling to identify altered signaling pathways after GPR17 silencing that are responsible for its effect on oligodendrocyte maturation.

2022-08-03 | GSE208409 | GEO

Transcriptomic analysis of undifferentiated and differentiated NMR OPCs.

Project description:Transcriptome of long-lived naked-mole rat (NMR) oligodendrocyte progenitor cells (OPCs) were compared with OPCs of other species.

2022-03-31 | E-MTAB-9160 | biostudies-arrayexpress

Gene expression analysis after GPR17 silencing in oligodendrocyte precursor cells (OPCs)

Project description:Gene expression analysis after GPR17 silencing in oligodendrocyte precursor cells (OPCs)

| PRJNA859818 | ENA

Direct Conversion of Fibroblasts into Oligodendrocyte Progenitor Cells

Project description:Many studies have already shown the reprogramming of somatic cells into other cell types such as neural stem cells, blood progenitor cells, and hepatocytes by inducing combinations of transcription factors. One of the recent development in cellular reprogramming is the direct reprogramming, that can change cell fate towards different lineages. This strategy provides an alternative to the use of pluripotent stem cells ruling out the concerns of tumorigenicity caused by undifferentiated cell populations. Here, we generated induced oligodendrocyte progenitor cells (iOPCs) from mouse fibroblasts by direct reprogramming. The generated iOPCs are homogenous, self-renewing, and multipotent. Once differentiated, the somatic stem cells exhibit morphological and molecular characteristics of oligodendrocyte progenitor cells (OPCs). Thus, we demonstrated that terminally differentiated somatic cells can be converted into functional iOPCs by induction of transcription factors offering a new strategies to cure myelin disorders. To identify the global gene expression profiles of iOPCs, we analyzed total 6 samples.

2015-11-02 | E-GEOD-52035 | biostudies-arrayexpress

Gene expression analysis after miR-125a-3p over-expression in oligodendrocyte precursor cells (OPCs)

Project description:MiR-125a-3p over-expression in OPCs impairs their differentiation into fully mature oligodendrocytes. We performed a whole microarray profiling to identify new miR-125a-3p direct targets and altered signaling pathways responsible for its the detrimental effect on oligodendrocyte maturation.

2021-01-21 | GSE143876 | GEO

Rapid and robust generation of functional oligodendrocyte progenitor cells from epiblast stem cells

Project description:Stem cell biology has garnered much attention due to its potential to impact human health through disease modeling and cell replacement therapy. This is especially pertinent to myelin-related disorders such as multiple sclerosis and leukodystrophies where restoration of normal oligodendrocyte function could provide an effective treatment. Progress in myelin repair has been constrained by the difficulty in generating pure populations of oligodendrocyte progenitor cells (OPCs) in sufficient quantities. Pluripotent stem cells theoretically provide an unlimited source of OPCs but significant advances are currently hindered by heterogeneous differentiation strategies that lack reproducibility. Here we provide a platform for the directed differentiation of pluripotent mouse epiblast stem cells (EpiSCs) through a defined series of developmental transitions into a pure population of highly expandable OPCs in ten days. These OPCs robustly differentiate into myelinating oligodendrocytes both in vitro and in vivo. Our results demonstrate that pluripotent stem cells can provide a pure population of clinically-relevant, myelinogenic oligodendrocytes and offer a tractable platform for defining the molecular regulation of oligodendrocyte development, drug screening, and potential cell-based remyelinating therapies. 6 total samples were analyzed. Pluripotent epiblast stem cells (EpiSCs) were differentiated to patterned neural rosettes, oligodendrocyte progenitor cells (OPCs), and oligodendrocytes. OPCs and oligodedrocytes were analyzed at two separate passages (3 and 11).

2011-10-02 | E-GEOD-31562 | biostudies-arrayexpress

Transcription factor–mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells

Project description:Cell-based therapies for myelin disorders, such as multiple sclerosis and leukodystrophies, require technologies to generate functional oligodendrocyte progenitor cells. Here we describe direct conversion of mouse embryonic and lung fibroblasts to ‘induced’ oligodendrocyte progenitor cells (iOPCs) using sets of either eight or three defined transcription factors. iOPCs exhibit a bipolar morphologyical and global gene expression profile molecular features consistent with bona fide OPCs. They can be expanded in vitro for at least five passages while retaining the ability to differentiate into induced multiprocessed oligodendrocytes. When transplanted to hypomyelinated mice, iOPCs are capable of ensheathing host axons and generating compact myelinmyelinating axons both in vitro and in vivo. Lineage conversion of somatic cells to expandable iOPCs provides a strategy to study the molecular control of oligodendrocyte lineage identity and may facilitate neurological disease modeling and autologous remyelinating therapies. 6 total samples were analyzed. MEFs were either untreated or infected with inducible lentiviral vectors containing the open reading frames of transcription factors. Samples were compared to bona fide OPCs.

2013-04-14 | E-GEOD-45440 | biostudies-arrayexpress

The role of CD44 in the naked-mole rat oligodendrocyte progenitor cells

Project description:The role of CD44 in naked-mole rats (NMR) oligodendrocyte progenitor cells (OPCs) were evaluated by comparing the transcriptome of control and CD44-knockdown NMR OPCs.

2022-03-31 | E-MTAB-9166 | biostudies-arrayexpress

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