Project description:Human placentas were collected following birth and formalin fixed paraffin embedded. Villitis of unknown etiology (VUE) cases were microdissected by laser capture microscopy for areas of high and low immune cell inflitration. RNA was extracted by Qiagen's RNeasy and run on Affymetrix U133 plus 2.0 arrays.
Project description:Placental villous tissue from women at term presenting various degrees of villitis of unknown etiology were analysed using Affymetrix microarrays. The severity of inflammation was graded using four levels: 0(no inflammation), 1(mild inflammation - multifocal low grade villitis), 2(moderate inflammation - patchy high grade villitis), and 3(severe inflammation - diffuse high grade villitis).
Project description:GeoMX digital spatial profiling is used to explore gene expression patterns in inflammatory and other pathways in chronic intervillositis of unknown etiology (CIUE).