Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis reveals significant differences between primary plasma cell leukemia and multiple myeloma even when sharing a similar genetic background

ABSTRACT: Transcriptome analysis reveals significant differences between primary plasma cell leukemia and multiple myeloma even when sharing a similar genetic background

PROVIDER: PRJNA542924 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome analysis reveals significant differences between primary plasma cell leukemia and multiple myeloma even when sharing a similar genetic background

Project description:Primary plasma cell leukemia (pPCL) is a highly aggressive plasma cell dyscrasia characterized by short remissions and very poor survival. Deletions of 17p13 are much more frequent in pPCL than in multiple myeloma (MM). Although the 17p deletion is associated with poor outcome and extramedullary disease in MM, its presence does not confer the degree of aggressiveness observed in pPCL. The comprehensive exploration of the transcriptome, including isoform expression and RNA splicing events, may provide novel information about biological differences between the two diseases carrying a 17p deletion. Transcriptomic studies were carried out with the Human Transcriptome Array in nine newly diagnosed pPCL and ten MM samples, all of which harbored the 17p deletion. Unsupervised cluster analysis clearly distinguished pPCL from MM samples. Indeed, 3,584 genes and 20,033 isoforms were found to be deregulated between pPCL and MM, most of which were underexpressed in pPCL. Pathway enrichment analysis at the gene and isoform levels revealed seven overlapping pathways mainly related to mRNA processing. There were 2,727 significantly deregulated isoforms of non-differentially expressed genes. Strangely enough, significant differences were observed in the expression of spliceosomal machinery components between pPCL and MM, in respect of the gene and isoform expression, and of the alternative splicing events. Furthermore, most of the deregulated exons have binding sites for most serine/arginine-rich proteins. In summary, transcriptome analysis revealed significant differences in the relative abundance of isoforms between pPCL and MM, even when they both had the 17p deletion. Importantly, the mRNA processing pathway including RNA splicing machinery emerged as one of the most remarkable mechanisms underlying the biological differences between the two entities.

2019-10-29 | GSE131216 | GEO

Transcriptome analysis reveals significant differences between primary plasma cell leukemia and multiple myeloma even when sharing a similar genetic background.

Project description:Primary plasma cell leukemia (pPCL) is a highly aggressive plasma cell dyscrasia characterised by short remissions and very poor survival. Although the 17p deletion is associated with poor outcome and extramedullary disease in MM, its presence does not confer the degree of aggressiveness observed in pPCL. The comprehensive exploration of isoform expression and RNA splicing events may provide novel information about biological differences between the two diseases. Transcriptomic studies were carried out in nine newly diagnosed pPCL and ten MM samples, all of which harbored the 17p deletion. Unsupervised cluster analysis clearly distinguished pPCL from MM samples. In total 3584 genes and 20033 isoforms were found to be deregulated between pPCL and MM. There were 2727 significantly deregulated isoforms of non-differentially expressed genes. Strangely enough, significant differences were observed in the expression of spliceosomal machinery components between pPCL and MM, in respect of the gene, isoform and the alternative splicing events expression. In summary, transcriptome analysis revealed significant differences in the relative abundance of isoforms between pPCL and MM, even when they both had the 17p deletion. The mRNA processing pathway including RNA splicing machinery emerged as one of the most remarkable mechanisms underlying the biological differences between the two entities.

| S-EPMC6868169 | biostudies-literature

CREST-E dbGaP Data Sharing

Project description:CREST-E dbGaP Data Sharing

| PRJNA419023 | ENA

CREST-E dbGaP Data Sharing

Project description:CREST-E dbGaP Data Sharing

| PRJNA419024 | ENA

ESBL-sharing

Project description:Healthy companion animals and owners colonisation and sharing of ESBL/AmpC-producing Enterobacterales, Portugal and United Kingdom

| PRJEB51686 | ENA

Bronchoalveolar lavage, sputum, background

Project description:Bronchoalveolar lavage, sputum, background Metagenome

| PRJNA1003499 | ENA

Pluripotent embryonic stem cells and multipotent adult germline stem cells reveal similar transcriptomes including pluripotency-related genes

Project description:DNA microarray analysis was performed with mouse multipotent adult germline stem cells (maGSCs) and embryonic stem cells (ESCs) from different genetic backgrounds cultured under standard ESC culture conditions and under differentiation-promoting conditions by the withdrawal of Leukemia Inhibitory Factor (LIF) and treatment with Retinoic Acid (RA). The analyzed undifferentiated cell lines are very similar based on their global gene expression pattern and show 97-99% identity dependent on the analyzed background. Only 621 genes are differentially expressed in cells derived from mouse 129SV-background, and 72 genes show differences in expression in cells generated from transgenic Stra8-EGFP/Rosa26-LacZ-background. Both maGSCs and ESCs express the same genes involved in the regulation of pluripotency, and even show no differences in the expression level of these genes. When comparing maGSCs with previously published signature genes of other pluripotent cell lines we could find that maGSCs share a very similar gene expression pattern with embryonic germ cells (EGCs). Also after differentiation of maGSCs and ESCs the transcriptomes of the cell lines are nearly identical which suggests that both cell types differentiate spontaneously in a very similar way. This is the first study comparing ESCs and a pluripotent cell line derived from an adult organism (maGSCs) on transcriptome level.

2010-07-12 | GSE22605 | GEO

Pluripotent embryonic stem cells and multipotent adult germline stem cells reveal similar transcriptomes including pluripotency-related genes

2010-07-12 | E-GEOD-22605 | biostudies-arrayexpress

Background mutagenesis during prolonged exposure

Project description:Background mutagenesis during prolonged exposure

| PRJEB34943 | ENA

Regulatory Sharing Between Estrogen Receptor α Bound Enhancers

Project description:Regulatory Sharing Between Estrogen Receptor α Bound Enhancers

| PRJNA613099 | ENA