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CD248 knockdown in in vitro differentiated adipocytes exposed to hypoxia

ABSTRACT: CD248 knockdown in in vitro differentiated adipocytes exposed to hypoxia

PROVIDER: PRJNA544327 | ENA |

REPOSITORIES: ENA

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CD248 knockdown in in vitro differentiated adipocytes exposed to hypoxia

Project description:Human adipose tissue derived stem cells were differentiated to adipocytes in vitro. At the end of differentiation, cells were treated with siRNA targeting CD248 followed by exposure to 1% oxygen levels. Microarray analysis were performed to identify differentially regulated genes.

2019-05-23 | GSE131667 | GEO

Secretome analysis of hypoxia-induced 3T3-L1 adipocytes uncovers novel proteins potentially involved in obesity

Project description:In the obese state, as adipose tissue expands, adipocytes become hypoxic and dysfunctional, leading to changes in the pattern of secreted proteins. To better understand the role of hypoxia in the mechanisms linked to obesity, we comparatively analyzed the secretome of differentiated 3T3-L1 adipocytes exposed to normoxia or hypoxia for 24 h. Proteins secreted into the culture media were precipitated using trichloroacetic acid and then digested by trypsin. Peptides were labeled by dimethyl labeling and analyzed by reversed phase nanoscale liquid chromatography coupled to a quadrupole Orbitrap mass spectrometer. Among factors downregulated in hypoxic conditions, we identified Decorin, a member of the leucine-rich proteoglycan family, Tissue Inhibitor of Metalloproteinase-2, Thrombospondin 1 and 2, all multifunctional proteins involved in extracellular matrix (ECM) homeostasis, and angiogenesis. Most findings were confirmed by expression studies of the relative genes in parallel experiments in vitro, in differentiated 3T3-L1 adipocytes, and in vivo, in fat tissues from obese vs. lean rats. Our observations are compatible with the concept that hypoxia may be an early trigger for ECM remodeling and angiogenesis in adipose tissue.

2018-02-27 | PXD006820 | Pride

Transcriptome analysis of human pancreatic preadipocytes and in vitro differentiated adipocytes

Project description:The aim of this study is to investigate the impact of the metabolic status on the transcriptome of isolated preadipocytes and in vitro differentiated adipocytes. We identified 38654 transcripts in pancreatic fat cells. We report that preadipocyte differentiation increased the abundance of mRNA levels of proteins related to adipogenesis and lipid metabolism. These changes in the transcriptome were absent or less pronounced in fat cells obtained from patients with prediabetes and type 2 diabetes. Meanwhile, mRNA levels of proteins involved in excellular matrix remodeling, angiogenesis and cytoskeleton organization were less abundant in adipocytes of all metabolic groups.

2021-09-22 | GSE169514 | GEO

Effects of differentiated hMADS adipocytes on BT474 and SKBR3 breast cancer cells

Project description:Analysis of gene expression levels of HER2-positive breast cancer cells exposed to the conditioned medium from adipocytes. The hypothesis tested in the present study was that adipocytes secrete factors that induce the resistance of cancer cells to antibody-dependent cellular cytotoxicity mediated by trastuzumab. The results provide insight into the genes that may be involved in the adipocyte-induced cancer resistance to trastuzumab treatment. BT474 cells or SKBR3 cells were exposed to the conditioned medium (CM) from differentiated hMADS (#hMADS) or to the control medium for 2 h. Total RNA was extracted and analyzed. The experiment was performed in triplicate.

2015-04-01 | E-GEOD-52660 | biostudies-arrayexpress

Hypoxia-induced modulation of gene expression in human adipocytes - A microarray study

Project description:Obesity is characterised by expansion of white adipose tissue, accompanied by an inflamatory response. It has been proposed that the inflammatory state observed may be linked to relative hypoxia in clusters of adipocytes distant from vasculature. Adipose tissue hypoxia has been observed in both animal models and human studies in obesity, and has been linked to insulin resistance. This study has used Agilent whole-genome microarrays to examine the effects of acute hypoxia on the global gene expression in human adipocytes. Human adipocytes (Zen-bio cells) were incubated in hypoxic conditions (1% O2) for 24 h. Control human adipocytes were incubated under normoxic conditions (21% O2). Eight biological replicates were prepared for each experimental condition, and RNA was pooled from two biological replicates, giving a total of n=4 array replicates.

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Effect of YBX1 knockdown on gene expression of differentiated C3H10T1/2 brown adipocytes

Project description:To investigate the function of YBX1 in the regulation of brown adipose aging, differentiated C3H10T1/2 brown adipocytes were transfected with specific siRNAs targeting YBX1 or control siRNA. Total RNA was extracted at 48h after transfection to performed gene expression profiling analysis by high throughput RNA-seq.

2024-01-04 | GSE222103 | GEO

RNAseq in JARID2 siRNA-treated in vitro differentiated human primary adipocytes

Project description:RNAseq in JARID2 siRNA-treated in vitro differentiated human primary adipocytes

| PRJNA690568 | ENA

Transcriptome analysis of human pancreatic preadipocytes and in vitro differentiated adipocytes

Project description:Transcriptome analysis of human pancreatic preadipocytes and in vitro differentiated adipocytes

| PRJNA716957 | ENA

Limited oxygen availability in standard cell culture alters metabolism and function in terminally differentiated cells

Project description:<p>Cell culture is generally considered to be hyperoxic. However, the importance of cellular oxygen consumption is often underappreciated, with rates of oxygen consumption often sufficient to cause hypoxia at cell monolayers. We initially focused on cultured adipocytes as a terminally differentiated cell-type with substantial oxygen consumption rates to support diverse cellular functions. Under standard conditions, cultured adipocytes are hypoxic and highly glycolytic. Increasing oxygen diverted glucose flux toward mitochondria and resulted in thousands of gene expression changes that pointed toward alleviated physiological transcriptional responses to hypoxia. Phenotypically, providing more oxygen increased adipokine secretion and rendered adipocytes more sensitive to insulin and lipolytic stimuli. The functional benefits of increasing pericellular oxygen were transferable to other cellular systems including hPSC-derived hepatocytes and cardiac organoids. Our findings suggest that oxygen is limiting in many terminally-differentiated cell culture systems, and that controlling oxygen availability can improve the quality and translatability of cell models.</p>

2024-02-21 | MTBLS6677 | MetaboLights

Gene microarray of human adipocytes exposed to medium conditioned by inflamed adipose tissue

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2016-12-01 | GSE75710 | GEO

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