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RNAseq of renal dendritic cells from adult Clec9a-Cre Rosa-YFP mice

ABSTRACT: RNAseq of renal dendritic cells from adult Clec9a-Cre Rosa-YFP mice

PROVIDER: PRJNA544681 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9118920.fastq.gz	Fastqsanger.gz
	SRR9118930.fastq.gz	Fastqsanger.gz
	SRR9118940.fastq.gz	Fastqsanger.gz
	SRR9118921.fastq.gz	Fastqsanger.gz
	SRR9118931.fastq.gz	Fastqsanger.gz

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RNAseq of renal dendritic cells from adult Clec9a-Cre Rosa-YFP mice

Project description:We use bulk RNA sequencing of sorted cells to characterize the gene expression profiles of renal dendritic cell (DC) subsets, cDC1 and cDC2, as well as MHCII+CD64+ F4/80hi and MHCII+CD64+ CD11bhi cells. Splenic DCs and red pulp macrophages serve as reference populations for a macrophage-like or DC-like phenotype. By sorting YFP+/YFP- F4/80hi or YFP+/YFP- CD11bhi cells from Clec9a-Cre Rosa-YFP mice we aim to reveal transcriptional differences between YFP-labelled and unlabelled cells. We showed that F4/80hi cells resemble macrophages on a transcriptional level, despite their DC origin, and that renal CD11bhi cells are a transcriptionally unique subset. However, we were not able to reveal differences between YFP+ and YFP- populations.

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