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Transcriptomic analysis of effect of oncolytic measles virus (MV) on transformed and non-transformed bone marrow-derived mesenchymal stem cells (MSCs)

ABSTRACT: Transcriptomic analysis of effect of oncolytic measles virus (MV) on transformed and non-transformed bone marrow-derived mesenchymal stem cells (MSCs)

PROVIDER: PRJNA545099 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9134410.fastq.gz	Fastqsanger.gz
	SRR9134420.fastq.gz	Fastqsanger.gz
	SRR9134430.fastq.gz	Fastqsanger.gz
	SRR9134411.fastq.gz	Fastqsanger.gz
	SRR9134421.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of effect of oncolytic measles virus (MV) on transformed and non-transformed bone marrow-derived mesenchymal stem cells (MSCs)

Project description:The mechanisms by which oncolytic vaccine strains of measles virus exert their tumor selectivity are not well elucidated. The goal of this study is to compare the gene expression profiles of MV-resistant MSC cell line (hTERT) and MV-susceptible MSC cell line (5H) before and after infection with MV (24 hours post infection) Through RNA-seq we were able to identify genes that are potentially contribute to the resistant phenotype of hTERT cells compared to their malignant counterparts, 5H cells.

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Project description:Measles (MV) is an aerosol-transmitted virus that affects more than 10 million children each year and accounts for approximately 120,000 deaths. While it was long believed to replicate in the respiratory epithelium before disseminating, it was recently shown to initially infect macrophages and dendritic cells of the airways using the signaling lymphocytic activation molecule (SLAM, CD150) as receptor. These cells then cross the respiratory epithelium and ferry the infection to lymphatic organs where MV replicates vigorously. How and where the virus crosses back into the airways has remained unknown. However, certain carcinoma cell lines have been described to be either permissive for wt MV infection, or not (Leonard VHJ et al. 2008). Therefor, we decided to compare the expression pattern of permissive and non-permissive cell lines by micro array analysis. Based on functional analyses of surface proteins preferentially expressed on virus-permissive epithelial cell lines, we identified nectin-4 (poliovirus-receptor-like-4) as a candidate receptor. The dataset comprises 21 samples divided into seven sample groups each representing 3 biological replicates of one out of 7 cell lines.

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Project description:Viruses manipulate central machineries of host cells to their advantage. They prevent host cell antiviral responses in order to create a favourable environment for their survival and propagation. Measles virus (MV) encodes two non-structural proteins MV-V and MV-C, proposed to counteract the host interferon response and to regulate cell death pathways in various functional assays. Several molecular mechanisms underlining MV-V regulation of innate immunity and cell death responses have been proposed, whereas MV-C host protein partners are less studied. We suggest that some cellular factors that are controlled by MV-C protein during viral replication could be components of innate immunity and the cell death pathways. In order to determine which host factors are hijacked by MV-C, we captured both direct and indirect host protein partners of MV-C protein. For this we used a strategy based on recombinant viruses expressing tagged viral proteins followed by affinity purification and a bottom-up mass spectrometry analysis. A list of host proteins specifically interacting with MV-C protein in different cell lines was identified.

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