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LPS-induced genome-wide RELA binding sites and H3K27ac changes in phraryngeal epithelial cells

ABSTRACT: LPS-induced genome-wide RELA binding sites and H3K27ac changes in phraryngeal epithelial cells

PROVIDER: PRJNA545651 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9165402.fastq.gz	Fastqsanger.gz
	SRR9165403.fastq.gz	Fastqsanger.gz
	SRR9165404.fastq.gz	Fastqsanger.gz
	SRR9165405.fastq.gz	Fastqsanger.gz
	SRR9165406.fastq.gz	Fastqsanger.gz

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LPS-induced genome-wide RELA binding sites and H3K27ac changes in phraryngeal epithelial cells

Project description:Gram negative endotoxin Lypopolysaccharide (LPS) leads to a strong innate immune response through TLR4 signalling. This latter pathway activates cannonical NF-kB pathway, including its member RELA. Here, we want to investigate the RELA response in terms of DNA binding as well as epigenetic changes induced by LPS recognition.

2019-06-01 | GSE132018 | GEO

Changes in H3K27ac following LPS stimulation in pharyngeal epithelial cells

Project description:We report changes in H3K27ac following LPS stimulation in Detroit 562 cells. We were able to identified LPS-increased H3K27ac regions which correlated with RELA binding as well as gene up-regulation. This data set is relevant for airborne bacterial sensing as Detroit 562 cells are nasopharyngeal epithelial cells and LPS is a gram negative bacterial endotoxin.

2018-09-24 | GSE104635 | GEO

LPS-induced RELA binding, H3K27ac changes and gene expression in pharyngeal epithelial FaDu cells

Project description:LPS-induced RELA binding, H3K27ac changes and gene expression in pharyngeal epithelial FaDu cells

| PRJNA545639 | ENA

Cut and run profiling of RELA binding sites in primary human proximal tubular cells

Project description:We performed cut and run assay to identify RELA binding sites in human primary proximal tubular cells

2024-07-31 | GSE252109 | GEO

PCR array of inflammatory cytokines and receptors in mouse lungs with NF-kappaB RelA mutant in alveolar epithelial cells during LPS stimulation

Project description:A murine model of RelA mutated throughout the alveolar epithelium was generated. Mice were anesthetized and intratracheally instilled with 50μg LPS into the left lung lobe. Mice were euthanized after 6 hours after instillation, and left lung lobes were collected to isolate RNA. We used SABioscience Mouse Inflammatory Cytokines and Receptors PCR Array to evaluate whether the expressions of lung cytokines and receptors during LPS stimulation are dependent on alveolar epithelial NF-κB RelA or not.

2012-08-03 | GSE38398 | GEO

PCR array of inflammatory cytokines and receptors in mouse lungs with NF-kappaB RelA mutant in alveolar epithelial cells during LPS stimulation

Project description:A murine model of RelA mutated throughout the alveolar epithelium was generated. Mice were anesthetized and intratracheally instilled with 50M-NM-<g LPS into the left lung lobe. Mice were euthanized after 6 hours after instillation, and left lung lobes were collected to isolate RNA. We used SABioscience Mouse Inflammatory Cytokines and Receptors PCR Array to evaluate whether the expressions of lung cytokines and receptors during LPS stimulation are dependent on alveolar epithelial NF-M-NM-:B RelA or not. qPCR gene expression profiling. RNA were collected from six different mouse lungs in each genotype (wild-type and alveolar epithelial RelA mutant). Equal amount of RNA (200ng) was pooled from six different mouse lungs, and 1M-NM-<g of total RNA (1.2M-NM-<g) was used to the PCR array.

2012-08-03 | E-GEOD-38398 | biostudies-arrayexpress

Genome-wide RELA binding sites in response to 5 microbial stimuli in Detroit562 cells [ChIP-seq]

Project description:NFKB is a family of transcription factors (TF), which are master regulators of the innate immune system. It is activated downstream of pathogen recognition receptors after ligand binding and regulates the expression of antimicrobials, cytokines and chemokines, thus helping to fight infections as well as recruiting the adaptive immune system. Although NFKB responds to a wide variety of signals, the processes in which stimulus-specificity is attained are still unclear. Here, we characterized the response of one of the NFKB members, RELA, to five stimuli mimicking infection in nasopharyngeal epithelial cells. When comparing RELA genome-wide binding sites , we detected most of them to be overlapping among the different stimulations. Yet, we distinguished a minority of stimulus-specific RELA binding sites. Interestingly, some of these seemed to have a biological effect as they correlated with corresponding gene expression. Specifically, the response to Poly I:C mimicking viral dsRNA gave a distinct RELA profile binding the vicinity of antiviral genes. This group of binding sites was also enriched in Interferon Regulatory Factor motifs indicating that the interaction with more specialized TFs could contribute to stimulus-specific RELA activity. Furthermore, motif analysis revealed differences in the sequence of the NFKB motifs between certain stimulus-specific sets of peaks suggesting preferences for particular NFKB dimers in response to defined signals.

2018-01-30 | GSE91018 | GEO

Changes in H3K27ac following LPS stimulation in pharyngeal epithelial cells

Project description:Changes in H3K27ac following LPS stimulation in pharyngeal epithelial cells

| PRJNA413376 | ENA

LPS-induced gene expression changes in phraryngeal epithelial cells

Project description:Gram negative endotoxin Lypopolysaccharide (LPS) leads to a strong innate immune response through TLR4 signalling. This latter pathway activates cannonical NF-kB pathway, including its member RELA. Here, we want to investigate the gene expression response induced by LPS recognition.

2019-06-01 | GSE132019 | GEO

Genome-wide identification of H3K27ac & H3K4me3-binding sequences upon LPS & CSF1 stimuli in mouse oocytes

Project description:We found that oocytes could arrange IgG production against adverse enviromental stimuli. We want to use chip-seq (cut-and-tag kit) to identify how different stimuli (LPS, CSF1) impact the binding of H3K27ac & H3K4me3 to transcription-regulation sequences of IgG gene

2022-10-30 | GSE191278 | GEO

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