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Identification of a master regulator of differentiation in Toxoplasma

ABSTRACT: Identification of a master regulator of differentiation in Toxoplasma

PROVIDER: PRJNA546593 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10359503_1.fastq.gz	Fastqsanger.gz
	SRR10359503_2.fastq.gz	Fastqsanger.gz
	SRR10359504_1.fastq.gz	Fastqsanger.gz
	SRR10359504_2.fastq.gz	Fastqsanger.gz
	SRR10359505_1.fastq.gz	Fastqsanger.gz

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Identification of a Master Regulator of Differentiation in Toxoplasma.

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Project description:The transcription factor BFD1 is the master regulator of the Toxoplasma chronic-stage differentiation program, which is induced under alkaline stress. Prior work indicated that BFD1 transcriptionally activates five other putative RNA- and DNA-binding proteins during differentiation, suggesting potential secondary roles in shaping the Toxoplasma chronic-stage transcriptome. To describe the distinct genetic cohorts controlled by these factors, we generated individual knockdown strains for each candidate using the auxin inducible degron system (TIR1/AID) and examined the transcriptomic effects of their depletion under alkaline stress.

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