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Flavivirus induces and antagonizes antiviral RNA interference in both mammals and mosquitoes

ABSTRACT: Flavivirus induces and antagonizes antiviral RNA interference in both mammals and mosquitoes

PROVIDER: PRJNA550985 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9600890.fastq.gz	Fastqsanger.gz
	SRR9600885.fastq.gz	Fastqsanger.gz
	SRR9600886.fastq.gz	Fastqsanger.gz
	SRR9600887.fastq.gz	Fastqsanger.gz
	SRR9600888.fastq.gz	Fastqsanger.gz

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Flavivirus induces and antagonizes antiviral RNA interference in both mammals and mosquitoes

Project description:Mosquito-borne flaviviruses maintain life cycles in mammals and mosquitoes. RNA interference (RNAi) has been demonstrated as an anti-flavivirus mechanism in mosquitoes; however, whether and how flavivirus induces and antagonizes RNAi-mediated antiviral immunity in mammals remains unknown. Here we showed that NS2A of Dengue virus-2 (DENV2) act as a viral suppressor of RNAi (VSR). When NS2A-mediated RNAi suppression was disabled, the resulting mutant DENV2 induced Dicer-dependent production of abundant DENV2-derived siRNAs in differentiated mammalian cells. Importantly, VSR-disabled DENV2 showed severe replication defects in mosquito and mammalian cells, and mice, which were rescued by the deficiency of RNAi. Moreover, NS2As of multiple flaviviruses act as VSRs in vitro and during viral infection in both organisms. Overall, our findings demonstrate that antiviral RNAi can be induced by flavivirus, while flavivirus uses NS2A as bona fide VSR to evade RNAi in mammals and mosquitoes, highlighting the importance of RNAi in flaviviral vector-host life cycles.

2020-02-24 | GSE133284 | GEO

Flavivirus induces and antagonizes antiviral RNA interference in both mammals and mosquitoes.

Project description:Mosquito-borne flaviviruses infect both mammals and mosquitoes. RNA interference (RNAi) has been demonstrated as an anti-flavivirus mechanism in mosquitoes; however, whether and how flaviviruses induce and antagonize RNAi-mediated antiviral immunity in mammals remains unknown. We show that the nonstructural protein NS2A of dengue virus-2 (DENV2) act as a viral suppressor of RNAi (VSR). When NS2A-mediated RNAi suppression was disabled, the resulting mutant DENV2 induced Dicer-dependent production of abundant DENV2-derived siRNAs in differentiated mammalian cells. VSR-disabled DENV2 showed severe replication defects in mosquito and mammalian cells and in mice that were rescued by RNAi deficiency. Moreover, NS2As of multiple flaviviruses act as VSRs in vitro and during viral infection in both organisms. Overall, our findings demonstrate that antiviral RNAi can be induced by flavivirus, while flavivirus uses NS2A as a bona fide VSR to evade RNAi in mammals and mosquitoes, highlighting the importance of RNAi in flaviviral vector-host life cycles.

| S-EPMC7002134 | biostudies-literature

Cooler temperatures destabilize RNA interference and increase susceptibility of disease vector mosquitoes to viral infection

Project description:The impact of global climate change on the transmission dynamics of infectious diseases is the subject of extensive debate. The transmission of mosquito-borne viral diseases is particularly complex, with climatic variables directly affecting many parameters associated with the prevalence of disease vectors. While evidence shows that warmer temperatures often decrease the extrinsic incubation period of an arthropod-borne virus (arbovirus), exposure to cooler temperatures often predisposes disease vector mosquitoes to higher infection rates. RNA interference pathways are essential to antiviral immunity in the mosquito; however, few experiments have explored the effects of temperature on the RNAi machinery. Total small RNAs (miRNAs, siRNAs, piRNAs, etc.) were isolated and sequenced from the heads of sensor strain Aedes aegypti mosquitoes, or from the whole bodies of CHIKV-infected Aedes albopictus mosquitoes 8 hours post infection. Mosquitoes were grown at 18C or 28C in replicates of 1 (Ae. aegypti) or 3 (Ae. albopictus).

2013-08-13 | E-GEOD-46204 | biostudies-arrayexpress

A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes

Project description:A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes

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TMEM41B is a pan-flavivirus host factor

Project description:Flaviviruses pose a constant threat to human health. These RNA viruses are transmitted by the bite of infected mosquitoes and ticks and regularly cause outbreaks. To identify host factors required for flavivirus infection we performed full-genome loss of function CRISPR-Cas9 screens. Based on these results we focused our efforts on characterizing the roles that TMEM41B and VMP1 play in the virus replication cycle. Our mechanistic studies on TMEM41B revealed that all members of the Flaviviridae family that we tested require TMEM41B. We tested 12 additional virus families and found that SARS-CoV-2 of the Coronaviridae also required TMEM41B for infection. Remarkably, single nucleotide polymorphisms (SNPs) present at nearly twenty percent in East Asian populations reduce flavivirus infection. Based on our mechanistic studies we propose that TMEM41B is recruited to flavivirus RNA replication complexes to facilitate membrane curvature, which creates a protected environment for viral genome replication.

2020-11-24 | GSE162040 | GEO

A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes [S0]

Project description:A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes [S0]

| PRJNA749555 | ENA

A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes [S0]

Project description:We report that the mosquito small RNA expression level in mosquitoes following a sugar meal

2022-05-01 | GSE180776 | GEO

A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes [miRNA-seq]

Project description:We report the human small RNA expression levels in mosquitoes following a blood meal

2022-05-01 | GSE167346 | GEO

A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes [miRNA-seq]

Project description:A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes [miRNA-seq]