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A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes [miRNA-seq]

ABSTRACT: A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes [miRNA-seq]

PROVIDER: PRJNA704365 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13771119.fastq.gz	Fastqsanger.gz
	SRR13771120.fastq.gz	Fastqsanger.gz
	SRR13771121.fastq.gz	Fastqsanger.gz
	SRR13771122.fastq.gz	Fastqsanger.gz
	SRR13771123.fastq.gz	Fastqsanger.gz

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A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes

Project description:A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes

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A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes

Project description:This SuperSeries is composed of the SubSeries listed below.

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Transcription profiling of female Anopheles gambiae mosquitoes blood fed on mice infected with P. berghei and treated with chloroquine or untreated

Project description:The effect of chloroquine on mosquitoes transcript abundance was assayed by comparing gene expression between mosquitoes fed on a blood meal containing 50 mg/Kg of chloroquine and those that had fed on a normal blood meal. Pools of 50 midguts were dissected and were hybridize with MMC1 (or 20K) microarrays. <br><br>Anopheles gambiae female mosquitoes were blood fed on BALB/c mice infected with P. berghei and intraperitonally pre-treated with 50 mg/kg of chloroquine. As controls, mosquitoes were blood fed on untreated P. berghei infected mice.<br><br>Mosquitoes were collected 24 hours post-blood feeding and pools of 50 midguts were dissected and processed for hybridization with MMC1 (or 20K) microarrays.Two different biological experiments were performed for each treatment.

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