Project description:The aim of this study was to look for the changes at the transcriptome level caused by the same T-DNA cassette in three mutants of Basmati 370 with different phenotypes. The comparative analysis of differentially expressed genes was conducted individually and collectively for all three mutants. RNA from Basmati mutants OsPE, Osoc and Ossl grown under optimal conditions were analyzed in two different biological replications (A and B) making total six samples.
Project description:The aim of this study was to look for the changes at the transcriptome level caused by the same T-DNA cassette in three mutants of Basmati 370 with different phenotypes. The comparative analysis of differentially expressed genes was conducted individually and collectively for all three mutants.
Project description:The aim of this study was to identify candidate genes responsible for grain number per panicle between a pair of rice varieties (Pusa 1266 and Pusa Basmati 1) by combining QTL analysis with expression analysis. Microarray analysis of RNA extracted from the panicle primordia showed 2741 differentially expressed genes. The differentially expressed genes were shortened to 18 on the basis of their occurance in the QTL region (responsible for grain number regulation) detected in RIL population derived from Pusa 1266 and Pusa Basmati 1. RNA from the stage '0' panicle primordia of Pusa 1266 and Pusa Basmati 1 were analysed in two different biological replications (A and B) making total four samples