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Mouse diet study

ABSTRACT: Mouse diet study Raw sequence reads

PROVIDER: PRJNA552446 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR9639651.fastq.gz	Fastqsanger.gz
	SRR9639653.fastq.gz	Fastqsanger.gz
	SRR9639655.fastq.gz	Fastqsanger.gz
	SRR9639647.fastq.gz	Fastqsanger.gz
	SRR9639657.fastq.gz	Fastqsanger.gz

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Project description:Purpose: Successive paternal generations of unhealthy diet induce a fat mass increase. The goal of this study is to compare epididymal adipose tissue transcriptome profiling (RNA-seq) of mice fed either a control-Diet or a High-Fat-Diet for One or Five successive generations. The aim of the study is to identify differentially expressed RNA in epididymal adipose tissue of obese males which might be involved in the exacerbation of induced by the maintenance of High Fat Diet feeding for 5 successive generations. Methods: Epididymal adipose tissue mRNA profiles of 18-months-old mice fed either a control-Diet or a Western-Diet for One or Five successive generations were generated by deep sequencing, in triplicate, using Illumina GAIIx. The sequence reads that passed quality filters were quantitavely analyzed. Reads abundance was evaluated for each gene followed by annotation versus mouse GTF by using the featureCounts function.The R package Edger was used in order to normalize the reads and to identify differentially expressed (DE) genes. Results: RNA-seq data revealed a specific enrichment in gene related to metabolic syndrome traits in male mice obtained after successive paternal generations of Western-Diet.

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