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Illumina sequencing of PCR products derived from TBLV-induced tumors in the presence and absence of Rem

ABSTRACT: Illumina sequencing of PCR products derived from TBLV-induced tumors in the presence and absence of Rem

PROVIDER: PRJNA554252 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR9674370_1.fastq.gz	Fastqsanger.gz
	SRR9674370_2.fastq.gz	Fastqsanger.gz
	SRR9674371_1.fastq.gz	Fastqsanger.gz
	SRR9674371_2.fastq.gz	Fastqsanger.gz
	SRR9674366_1.fastq.gz	Fastqsanger.gz

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Illumina sequencing of PCR products derived from TBLV-induced tumors in the presence and absence of Rem

Project description:Sequencing of portions (polymerase, envelope, and part of the 3’ LTR) of the TBLV retroviral genome were recovered by PCR amplification from tumors induced with or without Rem expression. PCR products from the mouse Gapdh gene were obtained as a control for acquisition of mutations during PCR and library preparation.

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Project description:We performed a PCR array of 84 ECM-related genes using RNA obtained from dermal fibroblasts stimulated presence or absence of IL-12, IL-23 or IL-27. The effects of IL-12, -23 or -27 on COL1A2 mRNA expression were less than 2-fold, as compared with untreated cells.

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Project description:We examined the effect of IL-17 signaling pathway on extracellular matrix (ECM) expression and the involvement of IL-17 signaling pathway in pathogenesis of SSc. To identify differences in the expression pattern of ECM genes in IL-17A- or IL-17F-treated cells, we performed PCR array analysis, consisting of 84 ECM-related genes. Normal human dermal fibroblasts were cultured until they were confluent, and then stimulated with IL-17A or IL-17F for 12 hours, and total RNA was extracted. A mixture of equal amounts of mRNAs from three normal fibroblasts was prepared in the presence or absence of IL-17A or -17F, and mRNA expression profile was evaluated using PCR Array.

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