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ABSTRACT: Adult granulosa cell tumor (AGCT) RNA-seq

PROVIDER: PRJNA555182 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9701540_1.fastq.gz	Fastqsanger.gz
	SRR9701540_2.fastq.gz	Fastqsanger.gz
	SRR9701550_1.fastq.gz	Fastqsanger.gz
	SRR9701550_2.fastq.gz	Fastqsanger.gz
	SRR9701531_1.fastq.gz	Fastqsanger.gz

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Ovarian granulosa cell tumor and normal granulosa cells transcriptomes

Project description:RNA was extracted from normal human granulosa cells from IVF patients (hGC1 and hGC2 samples) and from adult-type ovarian granulosa cell tumor samples (H1, H8, H20, H23, H24, H28, H30, H33, H4, H18) as described in Jamieson et al, 2010. RNA from all samples was linearly amplified using the Whole Transcriptome Amplification kit (Sigma), starting from 300ng of RNA, and with 12 amplifications cycles. cDNA was purified on columns and sent to the Nimblegen platform for hybridization and transcriptional profiling. The FOXL2 locus was gentoyped in tumor samples, and all samples were found positive for the recurrent somatic mutation p.Cys134Trp which is present in >95% of adult-type ovarian granulosa cell tumors (Shah et al, 2009).

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FOXO1/3 and PTEN Depletion in Granulosa Cells Promotes Ovarian Granulosa Cell Tumor Development

Project description:The Forkhead Box, FOXO1 and FOXO3, transcription factors regulate multiple functions in mammalian cells. Selective inactivation of the Foxo1 and Foxo3 genes in murine ovarian granulosa cells severely impairs follicular development and apoptosis causing infertility, and as shown herein, granulosa cell tumor (GCT) formation. Coordinate depletion of the tumor suppressor Pten gene in the Foxo1/3 strain enhanced the penetrance and onset of GCT formation A direct comparison of ovarian granulosa cells from wild type d25 and FOXO/PTEN knockout granulosa cell tumors.

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