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RNA-seq analysis of the transcriptional profile of SiHa cells with CNN3 interference.

ABSTRACT: RNA-seq analysis of the transcriptional profile of SiHa cells with CNN3 interference.

PROVIDER: PRJNA555795 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9722520_1.fastq.gz	Fastqsanger.gz
	SRR9722520_2.fastq.gz	Fastqsanger.gz
	SRR9722521_1.fastq.gz	Fastqsanger.gz
	SRR9722521_2.fastq.gz	Fastqsanger.gz
	SRR9722522_1.fastq.gz	Fastqsanger.gz

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RNA-seq analysis of the transcriptional profile of SiHa cells with CNN3 interference.

Project description:Transcriptome variation of SiHa cells transfected with si-CNN3 or si-NC were detected by RNA-seq.

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Transcriptome analysis of SiHa cells

Project description:This study assessed the transcriptional profile of SiHa cells. SiHa is a cervical cancer cell line with integrated HPV16, and was used as a model to study human gene expression in the context of integrated virus. Gene expression in SiHa, calculated by Cufflinks, was scored in windows around the locations of known viral integrations in patients or cell lines to determine if there was an association between gene expression and viral integration. We found that SiHa gene expression was higher near loci of integration for HPV18 vs. HPV16, cervical tissues vs. head and neck cancers, and cervical cancers vs. in vitro integrations. This study provides insight into the factors that may influence where viruses integrate in the human genome.

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RNA-Seq of H8, SiHa and KD HSPA9 SiHa cells

Project description:To investigate the different gens expression in the cervical immortalized cervical cells (H8 cells), cervical cancer cells (SiHa cells) and KD HSPA9 SiHa cells.

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miRNA expression profiles among SiHa, SiHa-tw2 and SiHa-shtw2

Project description:To further identify the most significantly changed miRNA resulting from the different expression levels of TWIST2 in cervical cancer cells, we used a miRNA microarray to identify the miRNA expression profiles among SiHa, SiHa-tw2 and SiHa-shtw2. The significantly deregulated miRNAs (changes of more than 2-fold in expression) included miR-23b-5p, miR-221-3p, miR-502-3p, miR-221-5p, miR-15a-5p, miR-1227, miR-93-5p and miR-4257.

2017-11-21 | GSE102706 | GEO

RNA-seq analysis of CaSki/SiHa/HeLa cell lines

Project description:We perfomed RNA-seq analysis using HPV16-postive CaSki and SiHa cells and HPV18-postive HeLa cells for HPV integration and gene expression analyses

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RNA-Seq of H8, SiHa and KD HSPA9 SiHa cells

Project description:RNA-Seq of H8, SiHa and KD HSPA9 SiHa cells

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Transcriptomic analysis of SiHa cells stably expressing shCtrl or shSYT7

Project description:GeneChip PrimeView Human PathArrayTM was performed using the SiHa cells-stably expressing shCtrl or shSYT7. To investigate the underlying mechanism of tumor-suppressive phenotypes of CESC with SYT7 knockdown, we used GeneChip PrimeView Gene Expression Array that could detect expression profiling of over 36,000 transcripts and variants using the SiHa-shCtrl and shSYT7-cDNAs

2022-04-11 | GSE200526 | GEO

The acRIP-seq of SiHa and KONAT10SiHa cells

Project description:We investigate genes that the different degrees of N4-acetylcytidine in the SiHa and Knockout NAT10 SiHa.

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The RIP-seq of SiHa and KOYTHDF3 cells

Project description:We find different genes that bind to the YTHDF3 by comparing the wild type SiHa with knockout YTHDF3 SiHa

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Altered global gene profile in KSHV-infected siHa cells

Project description:The Kaposi sarcoma-associated herpesvirus (KSHV) is the causative agent of Kaposi sarcoma (KS), the most common HIV/AIDS-associated tumor worldwide. Transmission routes of KSHV in the general population are poorly understood. Whereas sexual transmission appears to be common in homosexual men, the evidence for heterosexual transmission is less convincing. In fact, KSHVDNA sequences have been detected in the prostate, semen, and in the female genital tract. Persistent infection with high-risk human papillomavirus (HPV) is the major risk factor and is a requirement for the development of cervical cancer. However, it remains unknown the interaction between KSHV and HPV, and the contribution of KSHV to cervical cancer development and pathogenesis. In the present study, we used Illumina microarray to detect the global gene profile altered in KSHV-infected siHa cervical cancer cell-line containing integrated HPV16 genome.

2017-08-13 | GSE90039 | GEO

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