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Genome-wide CUT&RUN sequencing of H3K27me3 mark across different stages of T-cell development.

ABSTRACT: Genome-wide CUT&RUN sequencing of H3K27me3 mark across different stages of T-cell development.

PROVIDER: PRJNA556261 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9736920_1.fastq.gz	Fastqsanger.gz
	SRR9736920_2.fastq.gz	Fastqsanger.gz
	SRR9736921_1.fastq.gz	Fastqsanger.gz
	SRR9736921_2.fastq.gz	Fastqsanger.gz
	SRR9736916_1.fastq.gz	Fastqsanger.gz

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Genome-wide CUT&RUN sequencing of H3K27me3 mark across different stages of T-cell development.

Project description:We present CUT&RUN sequencing of H3K27me3 for hematopoietic progenitor cells at the following differentiation stages: 1) cKit+ hematopoietic progenitor cells from bone marrow, 2) monoallelic Bcl11b expressing DN2 progenitors, which have one active and one inactive Bcl11b allele and 3) biallelic Bcl11b expressing DN2 progenitors, which have both Bcl11b alleles active

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CUT&RUN-seq of H3K4me3, H3K27me3 and H3K27ac in mll2wt and mll2[Y2602A] mESC

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CUT & RUN H3K27me3 of Human Naïve and Primed Pluripotent Stem Cells with and without EZH2 inhibition.

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Identification of chromatin states during zebrafish gastrulation using CUT&RUN and CUT&Tag

Project description:Here we describe successful implementation of CUT&RUN for profiling protein-DNA interactions in zebrafish embryos. We apply modified a CUT&RUN method to generate high resolution maps of enrichment for H3K4me3, H3K27me3, H3K9me3, and RNA polymerase II during zebrafish gastrulation. Using this data, we identify a conserved subset of developmental genes that are enriched in both H3K4me3 and H3K27me3 during gastrulation, and we demonstrate the increased effectiveness of CUT&RUN for detecting protein enrichment at repetitive sequences with reduced mappability. Our work demonstrates the power of combining CUT&RUN with the strengths of the zebrafish system to better understand the changing embryonic chromatin landscape and its roles in shaping development.

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