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CUT & RUN analysis of H3K4me2 mark upon LSD1 inhibition

ABSTRACT: CUT & RUN analysis of H3K4me2 mark upon LSD1 inhibition

PROVIDER: PRJNA985560 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24968037_1.fastq.gz	Fastqsanger.gz
	SRR24968037_2.fastq.gz	Fastqsanger.gz
	SRR24968038_1.fastq.gz	Fastqsanger.gz
	SRR24968038_2.fastq.gz	Fastqsanger.gz
	SRR24968039_1.fastq.gz	Fastqsanger.gz

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CUT & RUN analysis of H3K4me2 mark upon LSD1 inhibition

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Project description:Long intergenic noncoding RNAs (lincRNAs) regulate chromatin states and epigenetic inheritance. Here we show that the lincRNA HOTAIR serves as a scaffold for at least two distinct histone modification complexes. A 5’ domain of HOTAIR binds Polycomb Repressive Complex 2 (PRC2) while a 3’ domain of HOTAIR binds the LSD1/CoREST/REST complex. The ability to tether two distinct complexes enables RNA-mediated assembly of PRC2 and LSD1, and coordinates targeting of PRC2 and LSD1 to chromatin for coupled histone H3 lysine 27 methylation and lysine 4 demethylation. Our results suggest that lincRNAs may serve as scaffolds by providing binding surfaces to assemble select histone modification enzymes, and thereby specify the pattern of histone modifications on target genes. Coordinate loss of SUZ12 and LSD1 occupancy caused by HOTAIR knockdown were concentrated in proximal promoters of HOXD genes. These regions correspondingly lost H3K27me3 and gained H3K4me2, the respective histone methylation products of PRC2 and LSD1 complexes. Comparison occupancy of LSD1 and SUZ12 of siGFP and siHOTAIR foreskin fibroblasts on HOX tiling array. Human foreskin fibroblasts were transfected with siGFP or siHOTAIR. The cells were harvested and ChIP analysis with anti-H3K4me2, anti-LSD1 and anti-SUZ12 antibodies was performed.

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