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ABSTRACT: Digester associated 16S rRNA meta-profiling in Nile tilapia (Oreochromis niloticus) and Nile perch (Lates niloticus) from Lake Victoria basin in Uganda.

PROVIDER: PRJNA556641 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9833220_1.fastq.gz	Fastqsanger.gz
	SRR9833220_2.fastq.gz	Fastqsanger.gz
	SRR9833230_1.fastq.gz	Fastqsanger.gz
	SRR9833230_2.fastq.gz	Fastqsanger.gz
	SRR9833240_1.fastq.gz	Fastqsanger.gz

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Project description:Triple-negative breast cancer (TNBC) lacks therapeutic target and is difficult to treat. We report a cationic antimicrobial peptide (CAP), tilapia piscidin 4 (TP4), derived from Nile tilapia (Oreochromis niloticus), selectively toxic to TNBC. Here we aim to identify potential target in TNBC cell response to TP4 treatment by microarray study and to further address the role of TP4-resposive genes involved in TNBC cell death.

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Genome-wide microRNA screening in Nile tilapia reveals pervasive transcription of isomiRs, sex-biased arm switching and increasing complexity of expression throughout development

Project description:The elucidation of microRNA function and evolution depends on the identification and characterization of miRNA repertoire of strategic organisms, as the fast evolving cichlid fishes. Using RNA-seq and comparative genomics we carried out an in-depth report of miRNAs in Nile tilapia (Oreochromis niloticus). Our results enlarge vertebrate miRNAs collection and reveal a notable differential expression of miRNAs arms and isoforms influenced by sex and developmental life stage, providing a better picture of the evolutionary and spatiotemporal dynamics of miRNAs.

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