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Role of NuRD subunits CHD3 and CHD4 in human melanoma cells (ChIP-seq)

ABSTRACT: Role of NuRD subunits CHD3 and CHD4 in human melanoma cells (ChIP-seq)

PROVIDER: PRJNA556642 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9833107.fastq.gz	Fastqsanger.gz
	SRR9833108.fastq.gz	Fastqsanger.gz

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Role of NuRD subunits CHD3 and CHD4 in human melanoma cells

Project description:Role of NuRD subunits CHD3 and CHD4 in human melanoma cells

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Role of NuRD subunits CHD3 and CHD4 in human melanoma cells (RNA-seq)

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CHD3 and CHD4 coordinate gene expression programs to maintain β-cell function and identity in vivo

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Evaluating and comparing the Transcriptome of (human) Hek 293 based cells, expressing either CHD3 or CHD4

Project description:Purpose: Identifying target genes of the two human chromatin remodeling enzymes CHD3 and CHD4 Methods: see below in protocols Results: Libraries were sequenced on Illumina HiSeq2000 platform resulting in 37-71 Mio 50 bp paired-end reads per sample. We identified 16 (i) and 115 (ii) distinctly regulated genes when CHD3-GFP (i) or CHD4-GFP (ii) were overexpressed. Nine genes seem to be commonly regulated by CHD3 and CHD4. We successfully validated four genes from our RNA-seq via qPCR with two new (independent from those, used for RNA-seq) biological replicates. Conclusion: CHD3 and CHD4 regulate distinct genes.

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CHD3 and CHD4 coordinate gene expression programs to maintain β-cell function and identity in vivo [RNA-seq]

Project description:To establish the role of CHD3 and CHD4 on controlling gene expression programs in the beta cell, we generated a tamoxifen-inducible, beta-cell-specific knockout mouse models; control (MIP-CreERT; Chd3+/+; Chd4+/+; R26RtdTomato), Chd3Db (MIP-CreERT; Chd3f/f; Chd4+/+; R26RtdTomato) and Chd3/4Db (MIP-CreERT; Chd3f/f; Chd4f/f; R26RtdTomato)

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