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Comparative gene expression profiling of human primary endotheliocytes cultivated on polyurethane-based electrospun 3D matrices and natural decellularized vein

ABSTRACT: Comparative gene expression profiling of human primary endotheliocytes cultivated on polyurethane-based electrospun 3D matrices and natural decellularized vein

PROVIDER: PRJNA556726 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9841050.fastq.gz	Fastqsanger.gz
	SRR9841051.fastq.gz	Fastqsanger.gz
	SRR9841052.fastq.gz	Fastqsanger.gz
	SRR9841053.fastq.gz	Fastqsanger.gz
	SRR9841054.fastq.gz	Fastqsanger.gz

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Comparative gene expression profiling of human primary endotheliocytes cultivated on polyurethane-based electrospun 3D matrices and natural decellularized vein

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In search for materials able to be colonized by a normal endothelium: сharacterization and NGS gene expression profiling of human primary endotheliocytes cultivated on electrospun 3D matrices

Project description:Endothelization of the luminal surface of vascular grafts is required for their long-term functioning. In order to select the best surface we have cultivated HUVEC on different 3D matrices and assessed the efficacy of cell proliferation and the changes in the gene expression profiles. 3D matrices were produced by electrospinning from solutions of poly(D,L-lactide-co-glycolide) (PLGA), polycaprolactone (PCL) and blends of PCL with gelatin (PCL-Gl) and glutaraldehyde-treated PCL-GL (PCL-Gl-glu) in hexafluoroisopropanol. Gene expression profiling was executed using Illumina HiSeq2500 platform.

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