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Next Generation Sequencing unveils the difference between human TR3-56 cells from healthy and T1D subjects

ABSTRACT: Next Generation Sequencing unveils the difference between human TR3-56 cells from healthy and T1D subjects

PROVIDER: PRJNA556751 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9841323_1.fastq.gz	Fastqsanger.gz
	SRR9841323_2.fastq.gz	Fastqsanger.gz
	SRR9841324_1.fastq.gz	Fastqsanger.gz
	SRR9841324_2.fastq.gz	Fastqsanger.gz
	SRR9841325_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing unveils the difference between human TR3-56 cells from healthy and T1D subjects

Project description:Purpose: The goal of this study was the transcriptome high-throughput data analysis of TR3-56 cells isolated from peripheral blood of healthy and T1D subjects Methods: total RNA profile of human TR3-56 cells from healthy and T1D subjects (1. Healthy Subject, 2.Healthy Subject, 3.Healthy Subject; 4. T1D subject, 5. T1D subject, 6. T1D subject; deeep sequencing, using Ovation SoLo RNA-seq Library Preparation KIt (NuGen), Library were prepared for sequencing on NextSeq 500 (llumina technology) Results: Principal component analysis and unsupervised clustering showed that TR3-56 cells from healthy donors are transcriptionally different from TR3-56 cells isolated from T1D subjects. Conclusions: Several genes are differently regulated between TR3-56 cells from T1D and healthy donors.

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Project description:We used an array-based bioassay whereby serum is used to induce transcription in healthy “reporter” leukocytes. Serum samples were obtained from control-arm subjects enrolled in 3 clinical trials evaluating immunotherapies in T1D that were conducted by the Immune Tolerance Network. The included trials evaluated immune-modifying therapy in new-onset T1D, with similar trial timecourses, primary outcomes, and data and sample collection. Total RNA was isolated from the reporter cell line for gene expression analysis.

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Project description:Technical replicate testing was performed to determine the measurement precision of each analyte in the array-based bioassay. Serum from subjects (n=5) with T1D was used to induce transcription in healthy “reporter” leukocytes. Three independent serum aliquots per subject were thawed and used to stimulate separate wells of reporter cells. Total RNA was isolated from the reporter cell line for gene expression analysis.

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