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The transcriptional profile of Trichophyton rubrum co-cultured with human keratinocytes shows new insights about gene modulation by terbinafine.

ABSTRACT: The transcriptional profile of Trichophyton rubrum co-cultured with human keratinocytes shows new insights about gene modulation by terbinafine.

PROVIDER: PRJNA557431 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9861020_1.fastq.gz	Fastqsanger.gz
	SRR9861020_2.fastq.gz	Fastqsanger.gz
	SRR9861021_1.fastq.gz	Fastqsanger.gz
	SRR9861021_2.fastq.gz	Fastqsanger.gz
	SRR9861022.fastq.gz	Fastqsanger.gz

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The transcriptional profile of Trichophyton rubrum co-cultured with human keratinocytes shows new insights about gene modulation by terbinafine.

Project description:Purpose: Evaluate the effect of terbinafine in T. rubrum gene expression using a co-culture model in conjunction with RNA-seq Methods: T. rubrum and HaCat cells line were co-cultured in RPMI medium supplemented with 5% of fetal bovine serum and treated with 0.0162 µM of terbinafine.The co-culture was incubated for 24 h at 37ºC in a humidified atmosphere containing 5% CO2 , followed RNA by extraction of both organisms, libraries construction and sequence. Results: Our data demonstrated the modulation of several T. rubrum genes involved in the biosynthesis of ergosterol in addition to the target gene of terbinafine that is already known, genes encoding MFS- and ABC-type membrane transporters that are associated with the phenomenon of multidrug resistance, genes that have been discussed in the literature as possible novel targets for antifungal compounds and genes which do not have a known function in T. rubrum yet, but have been modulated in response to the drug. Conclusions: RNA-seq sequencing of the T. rubrum co-culture in the presence of terbinafine showed that several genes of the ergosterol biosynthesis cascade were repressed. We highlight the induction of transmembrane transporters, which may be associated with the efflux of this allylamine. In addition, other genes that could be involved in the pathogenicity of T. rubrum were also modulated in the presence of the drug. In addition, we observed the modulation of hypothetical genes with still unknown functions in T. rubrum but that possess orthologs in other dermatophytes.

2019-11-28 | GSE135095 | GEO

Trichophyton rubrum

Project description:RNA-seq of Trichophyton rubrum treated by terbinafine

| PRJNA933916 | ENA

Trichophyton rubrum strain:N42WT

Project description:Genome analysis of terbinafine-resistant strain of Trichophyton rubrum

| PRJDB16125 | ENA

Trichophyton rubrum germination study

Project description:Conidia are considered to be the primary cause of skin and nail infections by Trichophyton rubrum. A cDNA microarray containing 10112 ESTs was developed and used to estimate the relative gene expression levels and changes of gene expression during conidial germination in time-course experiments. Keywords: time course

2007-03-27 | GSE5083 | GEO

Trichophyton rubrum strain:CMCC T1a

Project description:RNA-seq of Trichophyton rubrum treated by terbinafine

| PRJNA276042 | ENA

Global transcription profiles of Trichophyton rubrum in response to Flucytosine

Project description:A cDNA microarray was constructed from the expressed sequence tags (ESTs) of different developmental stages, and transcriptional profiles of the responses to 5-Flucytosine were determined. Keywords: Expression profiling by array The expression profiles of Trichophyton rubrum treated with 5-Flucytosine were compared to those of Trichophyton rubrum without drug. Each treatment has three replicates.

2010-05-19 | E-GEOD-12861 | biostudies-arrayexpress

Dermatophytes CGH based on a Trichophyton rubrum cDNA microarray

Project description:A cDNA microarray was constructed from the expressed sequence tags (ESTs) of different developmental stages, and comparative genome hybridization based on microarray procedures were carried out. Dermatophyte species are classified into three genera: Epidermophyton, Microsporum, and Trichophyton. To determine the relationship between these three groups comparative genome hybridization were used in our experiment. Trichophyton rubrum genmic DNA was reference DNA and labelled by Cy3 while the other dermatophytes genomic DNA were test DNA and labelled by CY5. Test and reference DNA were co-hybridized with the T. rubrum cDNA microarray and the numbers of genes shared between each species and T. rubrum were determined. Keywords: Comparative Genomic Hybridization We used a Trichophyton rubrum cDNA microarray prepared in our lab through comparative genome hybridization of genomic DNA of 21 dermatophyte strains (belonging to 20 species) to elucidate the taxonomy and evolution profiles of 20 dermatophyte species. The numbers of genes shared between each species and T. rubrum were determined. Each strain DNA hybridized for 3 times. The slides were separated into three groups base on different datasets.

2010-05-03 | E-GEOD-7909 | biostudies-arrayexpress

Dermatophytes CGH based on a Trichophyton rubrum cDNA microarray

2010-05-04 | GSE7909 | GEO

Trichophyton rubrum

Project description:Trichophyton rubrum germination study

| PRJNA95383 | ENA

Transcriptional changes of the human pathogenic fungus Trichophyton rubrum related to dermatophyte-skin interaction

Project description:Trichophyton rubrum, an anthropophilic and cosmopolitan fungus, is the most common agent of superficial mycoses, causing rarely deep dermatophytosis in immunocompromised hosts.

2011-10-03 | GSE15532 | GEO

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