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An ontogenetic switch in the positive and negative selection of B cells [bone marrow and fetal liver]

ABSTRACT: An ontogenetic switch in the positive and negative selection of B cells [bone marrow and fetal liver]

PROVIDER: PRJNA557934 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9879320_1.fastq.gz	Fastqsanger.gz
	SRR9879320_2.fastq.gz	Fastqsanger.gz
	SRR9879321_1.fastq.gz	Fastqsanger.gz
	SRR9879321_2.fastq.gz	Fastqsanger.gz
	SRR9879322_1.fastq.gz	Fastqsanger.gz

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An ontogenetic switch in the positive and negative selection of B cells

Project description:An ontogenetic switch in the positive and negative selection of B cells

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An ontogenetic switch in the positive and negative selection of B cells [IgHEL]

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An ontogenetic switch in the positive and negative selection of B cells [bone marrow and fetal liver]

Project description:Developing B cells can differentiate into a variety of different subsets, but the mechanisms that determine these outcomes are incompletely understood, with theories based on separate lineages or the positive and negative selection by self-antigens. In particular, the development of the B-1 B cell subset arises mainly from precursors in early ontogeny and is subject to positive selection by self-antigens, whereas the follicular B-2 B cell subset arises later and is subject to negative control. In this study we show how a cell intrinsic switch from Lin28b to Let7 gene expression changes a B cell’s susceptibility from positive to negative selection during ontogeny. Ectopic expression of a Lin28b transgene in B cells restores the positive selection of autoreactive B-1 B cells by self-antigen in adult bone marrow. Analysis of antigen-specific immature B cells in early and late ontogeny identifies Lin28b-dependent genes associated with B-1 B cell development, including Arid3a and Bhleh41, and Lin28b-independent effects associated with the presence or absence of self-antigen. These findings identify cell intrinsic and extrinsic determinants of B cell fate during ontogeny and reconcile lineage and selection theories of B cell development. They explain how changes in the balance of positive and negative selection may be able to adapt to meet the immunological needs of an individual during its lifetime.

2020-02-26 | GSE135224 | GEO

An ontogenetic switch in the positive and negative selection of B cells [B cell subsets]

Project description:An ontogenetic switch in the positive and negative selection of B cells [B cell subsets]

| PRJNA559546 | ENA

An ontogenetic switch in the positive and negative selection of B cells [IgHEL]

2020-02-26 | GSE135648 | GEO

An ontogenetic switch in the positive and negative selection of B cells [B cell subsets]

2020-02-26 | GSE135649 | GEO

An ontogenetic switch drives the positive and negative selection of B cells.

Project description:Developing B cells can be positively or negatively selected by self-antigens, but the mechanisms that determine these outcomes are incompletely understood. Here, we show that a B cell intrinsic switch between positive and negative selection during ontogeny is determined by a change from Lin28b to let-7 gene expression. Ectopic expression of a Lin28b transgene in murine B cells restored the positive selection of autoreactive B-1 B cells by self-antigen in adult bone marrow. Analysis of antigen-specific immature B cells in early and late ontogeny identified Lin28b-dependent genes associated with B-1 B cell development, including Arid3a and Bhleh41, and Lin28b-independent effects are associated with the presence or absence of self-antigen. These findings identify cell intrinsic and extrinsic determinants of B cell fate during ontogeny and reconcile lineage and selection theories of B cell development. They explain how changes in the balance of positive and negative selection may be able to adapt to meet the immunological needs of an individual during its lifetime.

| S-EPMC7035474 | biostudies-literature

An ontogenetic switch in the positive and negative selection of B cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-02-26 | GSE135650 | GEO

Lin28b controls a neonatal to adult switch in B cell positive selection

Project description:Lin28b controls a neonatal to adult switch in B cell positive selection

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Macs isolated E-cadherin positive Fetal liver stem progenitor cells vs adult mouse liver (pooled)

Project description:The isolation of hepatic stem cells from the adult liver has not yet been achieved due to the lack of specific surface markers. To identify new surface markers for hepatic stem cells, we analyzed differences in the gene expression profile of fetal liver epithelial cells (FLEC) and adult liver by microarray technology. In contrast to previous microarray studies on fetal liver, we purified FLEC by immunomagnetic selection for E-cadherin to more than 90% to analyze their specific expression profile. Keywords: cell type comparison Three independent biological repeats of purified FLEC were compared with adult liver and for each analysis, a dye switch was included to control for preferential dye incorporation, using a total of 6 microarrays.

2010-06-10 | E-GEOD-6340 | biostudies-arrayexpress

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