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Microorganisms in anaerobic Biological treatment under the nitrate nitrogen load in the EGSB

ABSTRACT: Microorganisms in anaerobic Biological treatment under the nitrate nitrogen load in the EGSB Raw sequence reads

PROVIDER: PRJNA558133 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9899513_1.fastq.gz	Fastqsanger.gz
	SRR9899513_2.fastq.gz	Fastqsanger.gz
	SRR9899514_1.fastq.gz	Fastqsanger.gz
	SRR9899514_2.fastq.gz	Fastqsanger.gz
	SRR9899515_1.fastq.gz	Fastqsanger.gz

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Microorganisms in anaerobic Biological treatment under the nitrate nitrogen load in the EGSB

Project description:Microorganisms in anaerobic Biological treatment under the nitrate nitrogen load in the EGSB Raw sequence reads

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Microorganisms in anaerobic biological treatment in the EGSB

Project description:Microorganisms in anaerobic biological treatment in the EGSB Raw sequence reads (different carbon-to-nitrogen ratio)

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Microorganisms in anaerobic Biological treatment under different carbon and nitrogen ratios in the EGSB

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Enrichment of C3 Nitrate microorganisms

Project description:Microorganisms able to perform C3-driven nitrate reduction were enriched in a bioreactor

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Project description:Nitrate-dependent anaerobic propane and butane oxidation coupled to anaerobic ammonium oxidation

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Transcriptome response to different nitrogen sources and aerobic/anaerobic conditions in Hydrogenobacter thermophilus

Project description:Transcriptome profile of the Hydrogenobacter thermophilus TK-6 cells aerobically or anaerobically grown on ammonium, nitrate, or mix of ammonium and nitrate was determined by NimbleGen Eukaryotic Expression array (4x72K).

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Effects of two-component system NarQ/P deletion on gene expression of Actinobacillus pleuropneumoniae in nitrate-rich anaerobic growth

Project description:Nitrate metabolism is an adaptation mechanism used by many bacteria for survival under anaerobic environments. As a by-product of inflammation, nitrate is used by the intestinal bacterial pathogens to enable gut infection. However, the responses of bacterial respiratory pathogens to nitrate are less well understood. Actinobacillus pleuropneumoniae is an important bacterial respiratory pathogen of swine. Previous studies have suggested that adaptation of A. pleuropneumoniae to anaerobiosis is important for infection. In this work, A. pleuropneumoniae growth and pathogenesis in response to the nitrate were investigated. Nitrate significantly promoted A. pleuropneumoniae growth under anaerobic condition in vitro and lethality in mice. By using narQ and narP deletion mutants, and single-residue mutation complementary strains of ΔnarQ, the two-component system NarQ/P were confirmed to be critical for nitrate induced growth, with Arg50 in NarQ as an essential functional residue. Transcriptome analysis showed that nitrate up-regulated multiple energy-generating pathways, including nitrate metabolism, mannose and pentose metabolism and glycerolipid metabolism via the regulation of NarQ/P. Furthermore, narQ, narP and its target gene encoding the nitrate reductase Nap contributed to the pathogenicity of A. pleuropneumoniae. The Nap inhibitor tungstate significantly reduced the survival of A. pleuropneumoniae in vivo, suggesting that Nap is a potential drug target. These results give new insights into how the respiratory pathogen A. pleuropneumoniae utilizes alternative electron acceptor nitrate to overcome the hypoxia microenvironment, which can occur in the inflammatory or necrotic infected tissues.

2022-07-29 | GSE207497 | GEO

Nitrate and ammonia as nitrogen sources for deep subsurface microorganisms.

Project description:We investigated the N-utilizing bacterial community in anoxic brackish groundwater of the low and intermediate level nuclear waste repository cave in Olkiluoto, Finland, at 100 m depth using (15)N-based stable isotope probing (SIP) and enrichment with (14∕15)N-ammonium or (14∕15)N-nitrate complemented with methane. Twenty-eight days of incubation at 12°C increased the concentration of bacterial 16S rRNA and nitrate reductase (narG) gene copies in the substrate amended microcosms simultaneously with a radical drop in the overall bacterial diversity and OTU richness. Hydrogenophaga/Malikia were enriched in all substrate amended microcosms and Methylobacter in the ammonium and ammonium+methane supplemented microcosms. Sulfuricurvum was especially abundant in the nitrate+methane treatment and the unamended incubation control. Membrane-bound nitrate reductase genes (narG) from Polarimonas sp. were detected in the original groundwater, while Burkholderia, Methylibium, and Pseudomonas narG genes were enriched due to substrate supplements. Identified amoA genes belonged to Nitrosomonas sp. (15)N-SIP revealed that Burkholderiales and Rhizobiales clades belonging to the minority groups in the original groundwater used (15)N from ammonium and nitrate as N source indicating an important ecological function of these bacteria, despite their low number, in the groundwater N cycle in Olkiluoto bedrock system.

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Xanthobacteraceae bacterium Astr-EGSB

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nitrate induction - nlp mutants-Nitrate induction - nlp mutants

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2012-12-01 | GSE39261 | GEO

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