Project description:RAW cells,ABHD5-KD RAW cells or those macrophage-primed CT-26 cells were subjected to microarray assays.Each group contain 3 samples.
Project description:To determine the transcriptomic differences between CT-26 and Colon 26, we sequenced RNA from these cell lines as well as tumors isolated ex vivo from tumor bearing animals at sizes 100, 400 and 800 mm3
Project description:Swimming signficantly attenuates tumor growth of CT-26 cells in vivo, and up-regulated 715 transcripts expression, while down-regulated 629 transcripts, comparing to Control group.
Project description:we provide a novel immune repertoire and RNA-seq joint library construction method (IRJL), we used IRJL protocol to compare the transcriptomic and immune repertoire of CT-26 tumor model and normal mouse spleen. Overall, IRJL offers a greatly simplified, shortcut and cost-effective protocol with RNA-seq and immune repertoire.
Project description:In osteolytic tumor, bone resportion can be occured after bone metastasis. Bone metastasis of colorectal cancer can induce obvious bone destruction. Aberrant activation of osteoclasts and the precursors is responsible for tumor-induced osteolysis. The biological process of osteoclast precursors could be modified by different tumor microenvironment. Microarrays were used to detail the differentially expressed genes or non-coding RNAs between normal osteoclast precursors and osteoclast precursors treated by secreta from CT-26 cells.
Project description:RAW cells or ABHD5-KD RAW cells were subjected to microarray assays. Each group contain 3 samples. To explore the regulatory role of ABHD5 in metabolic pathways, RAW cells or ABHD5-KD RAW cells were subjected to microarray assays.