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Indentification of differentially expressed genes on tumor tissues of CT-26 cells after swimming

ABSTRACT: Indentification of differentially expressed genes on tumor tissues of CT-26 cells after swimming

PROVIDER: PRJNA628616 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11614651_1.fastq.gz	Fastqsanger.gz
	SRR11614651_2.fastq.gz	Fastqsanger.gz
	SRR11614652_1.fastq.gz	Fastqsanger.gz
	SRR11614652_2.fastq.gz	Fastqsanger.gz
	SRR11614653_1.fastq.gz	Fastqsanger.gz

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Indentification of differentially expressed genes on tumor tissues of CT-26 cells after swimming

Project description:Swimming signficantly attenuates tumor growth of CT-26 cells in vivo, and up-regulated 715 transcripts expression, while down-regulated 629 transcripts, comparing to Control group.

2022-03-09 | GSE149405 | GEO

In vitro and in vivo transcriptome analysis of CT-26 and Colon 26 tumor models

Project description:To determine the transcriptomic differences between CT-26 and Colon 26, we sequenced RNA from these cell lines as well as tumors isolated ex vivo from tumor bearing animals at sizes 100, 400 and 800 mm3

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Gene expression in ABHD5-KD RAW cells or RAW cell-primed CT-26 cells

Project description:RAW cells,ABHD5-KD RAW cells or those macrophage-primed CT-26 cells were subjected to microarray assays.Each group contain 3 samples.

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Expression data from CD115+ preosteoclasts stimulated by conditioned medium from CT-26 cells

Project description:In osteolytic tumor, bone resportion can be occured after bone metastasis. Bone metastasis of colorectal cancer can induce obvious bone destruction. Aberrant activation of osteoclasts and the precursors is responsible for tumor-induced osteolysis. The biological process of osteoclast precursors could be modified by different tumor microenvironment. Microarrays were used to detail the differentially expressed genes or non-coding RNAs between normal osteoclast precursors and osteoclast precursors treated by secreta from CT-26 cells.

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Project description:we provide a novel immune repertoire and RNA-seq joint library construction method (IRJL), we used IRJL protocol to compare the transcriptomic and immune repertoire of CT-26 tumor model and normal mouse spleen. Overall, IRJL offers a greatly simplified, shortcut and cost-effective protocol with RNA-seq and immune repertoire.

2023-11-15 | GSE247573 | GEO

In vitro and in vivo transcriptome analysis of CT-26 and Colon 26 tumor models

Project description:In vitro and in vivo transcriptome analysis of CT-26 and Colon 26 tumor models

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Expression data from CD115+ preosteoclasts stimulated by conditioned medium from CT-26 cells

Project description:Expression data from CD115+ preosteoclasts stimulated by conditioned medium from CT-26 cells

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Gene expression in ABHD5-KD RAW cells or RAW cell-primed CT-26 cells

Project description:Gene expression in ABHD5-KD RAW cells or RAW cell-primed CT-26 cells

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Profiling T cell repertoires and RNA sequencing from mouse CT-26 tumor model by TRJL

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Swimming down-regulates ovarian transcriptomic response of rainbow trout Oncorhynchus mykiss

Project description:Metabolic processes and sexual maturation closely interact during the long-distance reproductive migration of many fish species to their spawning grounds. In the present study, we have for the first time used exercise experimentally to investigate the effects on sexual maturation in rainbow trout. Pubertal autumn-spawning seawater-raised female rainbow trout Oncorhynchus mykiss (n=26; 50-cm, 1.5-kg) were rested or swum at a near optimal speed of 0.75 body-lengths per second in a 6,000 L swim-flume under natural reproductive conditions (16 °C fresh-water, starvation, 8h-light:16h-dark photoperiod). Fish were sampled after arrival and subsequently after 10 days (resting or swimming 307 km) and 20 days (resting or swimming 636 km). Ovarian development was significantly reduced in the swimmers. Analysis of the expression of key factors in the reproductive axis included pituitary kiss1-receptor, lh and fsh and ovarian lh-receptor, fsh-receptor, aromatase and vitellogenin-receptor (vtgr). Swimmers had lower pituitary lh and ovarian vtgr expression than resters. Furthermore, the number of late vitellogenic oocytes was lower in swimmers than in resters, probably resulting from the lower vtgr expression, and vitellogenin plasma levels were higher. Therefore, swimming exercise suppresses oocyte development possibly by inhibiting vitellogenin uptake. Transcriptomic changes that occurred in the ovary of exercised fish were investigated using a salmonid cDNA microarray platform. Protein biosynthesis and energy provision were among the sixteen functional categories that were all down-regulated in the ovary. Down-regulation of the transcriptomic response in the ovary illustrates the priority of energy reallocation and will save energy to fuel exercise. A swimming-induced ovarian developmental suppression at the start of vitellogenesis during long-term reproductive migration may be a strategy to avoid precocious muscle atrophy.

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