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Whole genome RNA-sequencing from root tips of Arabidopsis thaliana med16-2 mutant under High and Low phosphate conditions

ABSTRACT: Whole genome RNA-sequencing from root tips of Arabidopsis thaliana med16-2 mutant under High and Low phosphate conditions

PROVIDER: PRJNA560045 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9961191.fastq.gz	Fastqsanger.gz
	SRR9961191_1.fastq.gz	Fastqsanger.gz
	SRR9961191_2.fastq.gz	Fastqsanger.gz
	SRR9961192.fastq.gz	Fastqsanger.gz
	SRR9961192_1.fastq.gz	Fastqsanger.gz

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Whole genome RNA-sequencing from root tips of Arabidopsis thaliana med16-2 mutant under High and Low phosphate conditions

Project description:Total mRNA was extracted from the root tips (2-3 mm from the root apex) of wild-type plants (Col-0 accession) and med16-2 mutants grown under low and high phosphate conditions 4 days after germination, using and sequenced by RNA-seq methodology.

2020-10-07 | GSE135778 | GEO

Whole genome RNA-sequencing from root tips ofArabidopsis thalianalow-phosphate insensitive mutants under High and Low phosphate

Project description:Phosphate limitation constrains plant development in natural and agricultural systems. Under phosphate-limiting conditions plants activate genetic, biochemical and morphological modifications to cope with phosphate starvation. One of the morphological modifications that plants induce under phosphate limitation is the arrest of primary root growth and it is induced by the root tip contact with low phosphate media. The sensitive to proton rhizotoxicity (stop1) and aluminium activate malate transporter 1 (almt1) mutants of Arabidopsis thaliana continue primary root growth under in vitro Pi-limiting conditions, thus, to get insight into the molecular components that control primary root growth inhibition under low phosphate conditions we extracted and sequenced mRNA from the root tips (2-3 mm from the root apex) of wild-type plants (Col-0 accession) and low-phosphate-insensitive mutants almt1 and stop1 grown under low and high phosphate conditions 5 days after germination using an RNA-seq methodology.

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Whole genome RNA-sequencing from roots of Arabidopsis thaliana wild-type and stop1 exposed to low pH, low phosphate (Pi), Aluminum and Iron excess conditions.

Project description:Total mRNA was extracted from the root tips (10 mm from the root apex) of wild-type plants (Col-0 accession) and stop1 mutants grown 5 days after germination under optimum conditions and then transferred for 16 hours to low phosphate(Pi), low pH, Al and Fe excess mediums.

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Expression signatures of Arabidopsis thaliana roots under low or high phosphate conditions.

Project description:Expression signatures of Arabidopsis thaliana roots under low or high phosphate conditions.

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Quantitative proteomics analysis of soybean leaves under low and high phosphate conditions

Project description:Low phosphate (LP) availability is a critical limiting factor affecting soybean production. Soybean plants develop a series of strategies to adapt phosphate limitation condition. To know the underlying molecular mechanisms responsible for LP stress response. A label-free quantification (LFQ) analysis of soybean leaves grown under low and high phosphate condition was performed.

2021-09-09 | PXD023343 | Pride

Transcription profiling by array of Pseudomonas aeruginosa under phosphate deficient conditions

Project description:Phosphate is essential for all living organisms, participating in critical biochemical processes, being an essential component of the energy dynamics of cells and being a component of nucleic acids, phospholipids in membranes, and other biomolecules. Therefore, the ability to withstand conditions of phosphate starvation, making use of the available phosphate, is of great importance for cell survival. Microarrays were performed to study the transcriptional response of Pseudomonas aeruginosa under phosphate deficient conditions.

2012-12-01 | E-MTAB-1170 | biostudies-arrayexpress

Transcriptome profiling of Arabidopsis tac1 and lazy1 mutant shoot tips under normal and gravistimulated conditions

Project description:Transcriptome profiling of Arabidopsis tac1 and lazy1 mutant shoot tips under normal and gravistimulated conditions

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Functional genomic analysis of the SPL9 gene in Arabidopsis thaliana under low phosphate conditions

Project description:Functional genomic analysis of the SPL9 gene in Arabidopsis thaliana under low phosphate conditions

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Expression signatures of Arabidopsis thaliana roots under low or high phosphate conditions.

Project description:To cope with limiting phosphorus (P) availability, plants have evolved a series of mechanisms to recycle internal P sources and to acquire P from the soil. One of these mechanisms is the release of low-molecular weight carboxylates, such as malate, which helps to liberate phosphate desorbed to aluminum and iron oxides. As malate release into the rhizosphere and root apopplast also increases iron availability. To identity genes involved in this interaction, we investigated time-dependent changes in the transcriptome of Arabidopsis thaliana roots exposed to sufficient and deficient phoshate levels .

2023-11-15 | GSE217790 | GEO

Functional genomic analysis of the SPL9 gene in Arabidopsis thaliana under low phosphate conditions

Project description:Inorganic phosphate (Pi) is an essential nutrient, which is often served as a limiting factor in plant growth. It has been reported that SPL family members, such as SPL3, regulate Pi deficiency responses by controlling the expression of Pi deficiency responsive genes. To elucidate whether SPL9 respond to low phosphorus stress, we investigated the phenotypes and conduct RNA sequencing analysis in transgenic Arabidopsis thaliana with overexpressing SPL9 (R9) under conditions of both normal and low Pi availability. Compared with wild-type plants, R9 showed decreased anthocyanin accumulation and increased Pi contents in shoots under Pi deficiency. Through RNA-seq analysis compared with wild-type plants, we detected 217 genes significantly differentially expressed in conditions of Pi sufficiency, and 121 genes differentially expressed in conditions of Pi deficiency in R9 plants. Under Pi deficiency, these genes included multiple protein kinases, jasmonic acid response genes and genes related to salt stress responses. Genes associated with hydrolase and transferase activity were also differentially regulated by Pi deficiency, such as cytochrome P450 monooxygenases. Of particular note, the transcription factor AP2-EREBP and members of the bHLH family were among the most significantly differentially regulated genes identified under both Pi sufficient and Pi deficient conditions.

2021-09-03 | GSE183312 | GEO

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