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ABSTRACT: Heat stress affects fecal microbiome of primiparous sows

PROVIDER: PRJNA560987 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9999920_1.fastq.gz	Fastqsanger.gz
	SRR9999920_2.fastq.gz	Fastqsanger.gz
	SRR9999921_1.fastq.gz	Fastqsanger.gz
	SRR9999921_2.fastq.gz	Fastqsanger.gz
	SRR9999922_1.fastq.gz	Fastqsanger.gz

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Project description:Heat stress affects fecal microbial and metabolic alterations of primiparous sows during late gestation

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Intermittent suckling does not influence the gene expression of Day 9 embryos during early gestation in primiparous sows

Project description:Primiparous sows were randomly allocated to two treatments and were separated from piglets 8h daily from Day 21 of lactation companied with daily boar exposure for oestrus detection until weaning (Day 28). Gene expression of Day 9 embryos were compared between control sows (FE; sows artificially inseminated when in heat during lactation ) and Skip-a-Heat sows (SE; sows in heat during lactation and artificially inseminated on the following oestrus cycle). Stimulating lactational oestrus then two mating strategies were applied to primiparous sows; 1)FE; sows were in heat during lactation and received artificial insemination) and Skip-a-Heat sows (SE; sows were in heat during lactation and received artificial insemination at fallowing oestrus cycle).

2013-12-23 | E-GEOD-53576 | biostudies-arrayexpress

Intermittent suckling does not influence the gene expression of Day 9 embryos during early gestation in primiparous sows

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Project description:We found that low protein diet consumption resulted in decrease in the percentage of normal Paneth cell population in wild type mice, indicating that low protein diet could negatively affect Paneth cell function. We performed fecal microbiota composition profiling. Male mice were used at 4-5 weeks of age. Fecal samples were collected for microbiome analysis.

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Project description:Glucosamine affects reproductive performance, fecal microbial and metabolic alterations of primiparous sows with fixed-time artificial insemination during gestation

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Project description:We found that western diet consumption resulted in decrease in the percentage of normal Paneth cell population in wild type mice, indicating that western diet could negatively affect Paneth cell function. Subsequent generations of western diet consumption further reduced percentages of normal Paneth cell population. We performed fecal microbiota composition profiling. Male mice were used at 4-5 weeks of age. Fecal samples were collected for microbiome analysis.

2023-01-01 | E-MTAB-10995 | biostudies-arrayexpress

Project description:N-carbamylglutamate affects reproductive performance, fecal microbial and metabolic alterations of primiparous sows with fixed-time artificial insemination during gestation

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Effect of morphine treatment on microbiome in the context of microbial transplant.

Project description:Morphine causes microbial dysbiosis. In this study we focused on restoration of native microbiota in morphine treated mice and looked at the extent of restoration and immunological consequences of this restoration. Fecal transplant has been successfully used clinically, especially for treating C. difficile infection2528. With our expanding knowledge of the central role of microbiome in maintenance of host immune homeostasis17, fecal transplant is gaining importance as a therapy for indications resulting from microbial dysbiosis. There is a major difference between fecal transplant being used for the treatment of C. difficile infection and the conditions described in our studies. The former strategy is based on the argument that microbial dysbiosis caused by disproportionate overgrowth of a pathobiont can be out-competed by re-introducing the missing flora by way of a normal microbiome transplant. This strategy is independent of host factors and systemic effects on the microbial composition. Here, we show that microbial dysbiosis caused due to morphine can be reversed by transplantation of microbiota from the placebo-treated animals.

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Project description:Transcriptional profiling of Day 30 embryos (D30E) was performed. First parity sows were submitted to an ovulation-induction protocol, intermittent suckling (IS), during lactation. IS consisted of 8 h/d separation from their litters during the last 7d of a 28d lactation. During separation, sows received boar exposure. There were 3 treatments: control (C28, n=19), where piglets were weaned at D28 of lactation and were bred after weaning and two IS treatments: sows were either bred at their first induced estrus during lactation (IS21FE, n=18), or were “skipped” and bred at their second estrus (IS21SE, n= 17) which occurred after final weaning at D28. Sows were slaughtered and embryos were collected on D30 of gestation for DNA PCR sexing. Later, D30E from the same sex with similar weight were pooled for further microarray investigation. Stimulating lactational oestrus then two mating strategies were applied to primiparous sows. For the microarray experiment, three biological replicates (three sows) were chosen from each treatment group comparing control (C28) to either IS21FE or IS21SE. A pool of females and males D30E were chosen and pooled separately for each comparison.

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