Project description:Adult pike Esox lucius was caught in the end of summer (August, 2020) in Teletskoye Lake (Altai region, west Siberia, Russia, 51°79’10’’ N, 87°30’43’’E). Parasite (Triaenophorus crassus) was retrieved from the intestine of pike and immediately frozen in liquid nitrogen. After that, the sample was lyophilized and sent in ice (-20 °C) to the Group of mass spectrometry of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS (Moscow, Russia) where further analysis was performed.
Project description:The aim of this sequencing experiment was to make available liver tissue expression for selected fish species, northern pike (Esox lucius, Eluc), coho salmon (Oncorhynchus kisutch, Okis) and Arctic charr (Salvelinus alpinus, Salp), for comparative expression studies between the species. Samples in replicate of four were sacrificed according to protocols at each of the facilities from where samples were obtained. RNA was extracted from samples and Illumina TruSeq Stranded mRNA libraries were built. Sequencing was performed in two passes on an Illumina HiSeq2500, paired-end 125bp reads. Processed count tables per species as raw counts, FPKM, or TPM, were generated from read alignment to the NCBI genomes of the respective species using STAR and gene level counting using RSEM and NCBI gene annotation.
