Project description:To evaluate the effect of histamine on LPS induced proinflammatory state of purified monocytes from healthy adults and newborns, we performed a qPCR for 84 genes related to toll-like receptor (TLR) signalling
Project description:Human cardiovascular stem cells were isolated from adult (57-75 year old) and neonatal (<1 month old) atrial tissue. Cardiovascular stem cells were then cloned by single cell dilution and compared using sabiosciences cell development & differentiation miRNA PCR array. microRNA expression profiling by RT-PCR, 3 cardiac progenitor cell clones isolated from adults were run separately and results were pooled and compared to 8 neonatal cardiovascular progenitor cell clones that were run separately and pooled.