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Solanum lycopersicum

ABSTRACT: Transcriptome analysis in primed tomato seeds with enhanced longevity by heat shock treatment

PROVIDER: PRJNA562700 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10056916_1.fastq.gz	Fastqsanger.gz
	SRR10056916_2.fastq.gz	Fastqsanger.gz
	SRR10056917_1.fastq.gz	Fastqsanger.gz
	SRR10056917_2.fastq.gz	Fastqsanger.gz
	SRR10056918_1.fastq.gz	Fastqsanger.gz

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The composition and function of chaperone networks in Solanum lycopersicum explored by transcriptome sequencing and microarray meta-analysis

Project description:Heat shock proteins (Hsps) are molecular chaperones primarily involved in maintenance of protein homeostasis. Their function has been best characterized in heat stress (HS) response during which Hsps are transcriptionally controlled by heat stress transcription factors (Hsfs). The role of Hsfs and Hsps in HS-response in tomato was initially examined by transcriptome analysis using the Massive Analysis of cDNA Ends (MACE) method. Approximately 9.6% of all genes expressed in leaves are enhanced in response to HS, including a subset of Hsfs and Hsps. The underlying Hsp-Hsf networks with potential functions in stress responses or developmental processes were further explored by meta-analysis of existing microarray datasets. We identified clusters with differential transcript profiles with respect to abiotic stresses, plant organs and developmental stages. The composition of two clusters points toward two major chaperone networks. One cluster consisted of constitutively expressed plastidial chaperones and other genes involved in chloroplast protein homeostasis. The second cluster represents genes strongly induced by heat, drought and salinity stress, including HsfA2 and many stress-inducible chaperones, but also potential targets of HsfA2 not related to protein homeostasis. This observation attributes a central regulatory role to HsfA2 in controlling different aspects of abiotic stress response and tolerance in tomato. 2 samples

2014-08-01 | E-GEOD-56611 | biostudies-arrayexpress

The composition and function of chaperone networks in Solanum lycopersicum explored by transcriptome sequencing and microarray meta-analysis

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Project description:Global analysis of gene expression associated with germination in tomato seeds.

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Project description:Tomato root culture and Al treatment

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Project description:Alternative splicing in tomato pollen in response to heat stress

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Solanum lycopersicum Transcriptome or Gene expression

Project description:To characterize the PTI response of tomato and the effect of the delivery of a subset of effectors, we performed an RNA-seq analysis of tomato Rio Grande prf3 leaves challenged with either the flgII-28 peptide or the following bacterial strains: Agrobacterium tumefaciens GV2260, Pseudomonas fluorescens 55, Pseudomonas putida KT2440, Pseudomonas syringae pv. tomato (Pst) DC3000, Pst DC3000 deltahrcQ-U deltafliC and Pst DC3000 deltaavrPto deltaavrPtoB. NOTE: Samples in SRA were assigned the same sample accession. This is incorrect as there are different samples, hence âSource Nameâ was replaced with new values. Comment[ENA_SAMPLE] contains the original SRA sample accessions.

2013-08-21 | E-MTAB-4789 | biostudies-arrayexpress

Effect of RS2Z35 and RS2Z36 single and double knockout on gene expression and splicing during control and heat stress conditions in tomato (Solanum Lycopersicum) leaf tissue

Project description:To investigate the role of Serine/Arginine-rich proteins RS2Z35 and RS2Z36 in tomato heat stress response and thermotolerance, we established single and double knockouts of the respective splicing factor(s) in tomato We then performed gene expression and splicing analysis using data obtained from RNA-seq of wildtype, single knockout and double knockout plants under control and heat stress conditions (1 h 40°C)

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Project description:We evaluated the effect of a 2h pre-treatment at 38°C on the response of Arabidopsis thaliana 6d-old seedlings to a 2h heat shock at 43°C applied 24 h later. A shotgun proteomics approach using total protein extracts was used to compare the proteome of seedlings from four type of samples, using 4 replicates: C=Control (just before heat shock on day 7) P= Primed (on day 7, 22 h after priming performed on day 6) H= Heat shock (on day 7, 2h after heat shock) PH= Priming + heat shock (on day 7, for primed seedlings, 2h after heat shock).

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