Ontology highlight
ABSTRACT:
PROVIDER: PRJNA563229 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR10060508.fastq.gz | Fastqsanger.gz | |||
| SRR10060508_1.fastq.gz | Fastqsanger.gz | |||
| SRR10060508_2.fastq.gz | Fastqsanger.gz | |||
| SRR10060509_1.fastq.gz | Fastqsanger.gz | |||
| SRR10060509_2.fastq.gz | Fastqsanger.gz |
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Project description:Codonopsis pilosula, a kind of traditional Chinese medicine, possesses anti-aging effect. However, the anti-aging effect of Codonopsis pilosula on lung remains largely unknow and the exact molecular mechanism of Codonopsis pilosula anti-aging also needs to be further studied. Thus, we investigated the protective effect of Codonopsis pilosula on aging lung and the exact molecular mechanism of Codonopsis pilosula anti-aging. To test this hypothesis, we established the aging mice model, and then used Codonopsis pilosula to intervene. Microarray analysis and bioinformatics methods were used to comprehensively analyze lncRNA-miRNA-mRNA (ceRNA) network. Our results showed that the aging mice model was established. From the microarray analysis, 138 lncRNAs, 128 mRNAs and seven miRNAs significantly changed after aging, whereas, 282 lncRNAs, 283 mRNAs and 19 miRNAs were dysregulated after intervention of Codonopsis pilosula. KEGG pathway analysis was performed to explore the signaling pathways involved. Compared with the ceRNA network in aging mice and after intervention of Codonopsis pilosula, we found three mRNAs (Hif3a, Zbtb16, Plxna2) and one lncRNA (NONMMUT063872) associated with Codonopsis pilosula anti-aging and they were validated by qRT-PCR. Altogether, our results showed that Codonopsis pilosula has the protective effect on aging lung and the ceRNA network plays an important role in Codonopsis pilosula anti-aging.
2020-02-26 | GSE139875 | GEO
Project description:Codonopsis pilosula, a kind of traditional Chinese medicine, possesses anti-aging effect. However, the anti-aging effect of Codonopsis pilosula on lung remains largely unknow and the exact molecular mechanism of Codonopsis pilosula anti-aging also needs to be further studied. Thus, we investigated the protective effect of Codonopsis pilosula on aging lung and the exact molecular mechanism of Codonopsis pilosula anti-aging. To test this hypothesis, we established the aging mice model, and then used Codonopsis pilosula to intervene. Microarray analysis and bioinformatics methods were used to comprehensively analyze lncRNA-miRNA-mRNA (ceRNA) network. Our results showed that the aging mice model was established. From the microarray analysis, 138 lncRNAs, 128 mRNAs and seven miRNAs significantly changed after aging, whereas, 282 lncRNAs, 283 mRNAs and 19 miRNAs were dysregulated after intervention of Codonopsis pilosula. KEGG pathway analysis was performed to explore the signaling pathways involved. Compared with the ceRNA network in aging mice and after intervention of Codonopsis pilosula, we found three mRNAs (Hif3a, Zbtb16, Plxna2) and one lncRNA (NONMMUT063872) associated with Codonopsis pilosula anti-aging and they were validated by qRT-PCR. Altogether, our results showed that Codonopsis pilosula has the protective effect on aging lung and the ceRNA network plays an important role in Codonopsis pilosula anti-aging.
2020-02-26 | GSE139874 | GEO
Project description:We used wheat as rotational crop to assess the influence of continuous cropping on microbiome in Pinellia ternata rhizosphere and the remediation of rotational cropping to the impacted microbiota. Illumina high-throughput sequencing technology was utilized for this method to explore the rhizosphere microbial structure and diversity based on continuous and rotational cropping.
2016-12-10 | GSE91083 | GEO
Project description:Objective: To explore the molecular mechanism of Codonopsis pilosula in enhancing the immune function of aging mice, the expression profile of long-chain non-coding RNA (lncRNA), mRNA in spleen of mice was detected. Methods: An aging mouse model was established, the aging mice were treated with low, middle and high doses of Codonopsis pilosula, and the aging related indexes, spleen pathology and cellular ultrastructure were detected and analyzed. Then microarray analysis and bioinformatics methods were used to analyze the lncRNA and mRNA expression profile. GO enrichment analysis and KEGG pathway analysis were performed to analyze the differential genes between groups using KOBAS. Finally, the common lncRNAs and mRNAs in high-dose Codonopsis pilosula treatment group compared with the model group and the model group compared with the control group were selected for lncRNA-mRNA co-expression network construction, and real-time quantitative polymerase chain reaction (qRT-PCR) was used to verify the genes in the network. Results: The aging mouse model was successfully established. Codonopsis pilosula can improve the pathology and cellular ultrastructure of spleen in aging mice. Microarray analysis showed that 96 lncRNAs and 65 mRNAs changed significantly after aging, 623 lncRNAs and 435 mRNAs expressed abnormally after high-dose Codonopsis pilosula treatment. KEGG pathways involved in aging and high-dose Codonopsis pilosula treatment are mainly related to immunity, including allograft rejection, graft-versus-host disease, autoimmune thyroid disease, antigen processing and presentation and so on. The three mRNAs (Enpp6, Cped1, and Galnt15) in lncRNA-mRNA co-expression network may be related to Codonopsis pilosula improving the immunity of aging mice, which were verified by qRT-PCR. Conclusion: Codonopsis pilosula has a certain protective effect on the spleen of aging mice and the lncRNA-mRNA network may play an important role in enhancing the immunity of aging mice.
2022-05-17 | GSE150773 | GEO
Project description:Soil transplant serves as a proxy to simulate climate change in realistic climate regimes. Here, we assessed the effects of climate warming and cooling on soil microbial communities, which are key drivers in EarthM-bM-^@M-^Ys biogeochemical cycles, four years after soil transplant over large transects from northern (N site) to central (NC site) and southern China (NS site) and vice versa. Four years after soil transplant, soil nitrogen components, microbial biomass, community phylogenetic and functional structures were altered. Microbial functional diversity, measured by a metagenomic tool named GeoChip, and phylogenetic diversity are increased with temperature, while microbial biomass were similar or decreased. Nevertheless, the effects of climate change was overridden by maize cropping, underscoring the need to disentangle them in research. Mantel tests and canonical correspondence analysis (CCA) demonstrated that vegetation, climatic factors (e.g., temperature and precipitation), soil nitrogen components and CO2 efflux were significantly correlated to the microbial community composition. Further investigation unveiled strong correlations between carbon cycling genes and CO2 efflux in bare soil but not cropped soil, and between nitrogen cycling genes and nitrification, which provides mechanistic understanding of these microbe-mediated processes and empowers an interesting possibility of incorporating bacterial gene abundance in greenhouse gas emission modeling. Fifty four samples were collected from three soil types (Phaeozem,Cambisol,Acrisol) in three sites (Hailun, Fengqiu and Yingtan) along a latitude with reciprocal transplant; Both with and without maize cropping in each site; Three replicates in every treatments.
2013-10-30 | E-GEOD-51592 | biostudies-arrayexpress
Project description:To investigate the underlying mechanism that Codonopsis Pilosula anti-aging in gastrointestinal tract of aged mice induced by D-galactose.we integrated stomach and intestine tissues, and aimed to explore the underlying mechanism that Codonopsis Pilosula participates in protecting gastrointestinal structure and regulating gastrointestinal function by microarray and real-time quantitative polymerase chain reaction (qRT-PCR).
2020-12-31 | GSE140949 | GEO
Project description:proteomic analysis of continuous cropping response in Codonopsis tangshen Oliv
2022-07-15 | PXD035371 |
Project description:To unravel the molecular mechanisms underpinning Codonopsis pilosula drought stress tolerance, we conducted comprehensive comparative transcriptome and physiological analyses of two C. pilosula cultivars that had been exposed to drought treatment for four days.
2024-11-20 | GSE270657 | GEO
Project description:The experiment at three long-term agricultural experimental stations (namely the N, M and S sites) across northeast to southeast China was setup and operated by the Institute of Soil Science, Chinese Academy of Sciences. This experiment belongs to an integrated project (The Soil Reciprocal Transplant Experiment, SRTE) which serves as a platform for a number of studies evaluating climate and cropping effects on soil microbial diversity and its agro-ecosystem functioning. Soil transplant serves as a proxy to simulate climate change in realistic climate regimes. Here, we assessed the effects of soil type, soil transplant and landuse changes on soil microbial communities, which are key drivers in Earth’s biogeochemical cycles.
2016-02-04 | GSE77546 | GEO
Project description:Soil transplant serves as a proxy to simulate climate change in realistic climate regimes. Here, we assessed the effects of climate warming and cooling on soil microbial communities, which are key drivers in Earth’s biogeochemical cycles, four years after soil transplant over large transects from northern (N site) to central (NC site) and southern China (NS site) and vice versa. Four years after soil transplant, soil nitrogen components, microbial biomass, community phylogenetic and functional structures were altered. Microbial functional diversity, measured by a metagenomic tool named GeoChip, and phylogenetic diversity are increased with temperature, while microbial biomass were similar or decreased. Nevertheless, the effects of climate change was overridden by maize cropping, underscoring the need to disentangle them in research. Mantel tests and canonical correspondence analysis (CCA) demonstrated that vegetation, climatic factors (e.g., temperature and precipitation), soil nitrogen components and CO2 efflux were significantly correlated to the microbial community composition. Further investigation unveiled strong correlations between carbon cycling genes and CO2 efflux in bare soil but not cropped soil, and between nitrogen cycling genes and nitrification, which provides mechanistic understanding of these microbe-mediated processes and empowers an interesting possibility of incorporating bacterial gene abundance in greenhouse gas emission modeling.
2013-10-30 | GSE51592 | GEO