Project description:To find out differently expressed circRNAs during Orf virus infection of GSF cells and their potential roles in response to ORFV infection
Project description:Contagious ecthyma (Orf) is a contagious disease with worldwide distribution, caused by the epitheliotropic Orf virus (ORFV), a member of the genus Parapoxvirus. In the current study, we collected the oral mucosa tissues samples (T0, T3, T7 and T15) from sheep at 0, 3, 7 and 15 days post ORFV infection, respectively. To explore the changes of comprehensive transcriptome of host cells from oral mucosa tissues post ORFV infection, the RNA-seq transcriptome comparisons were performed on these samples. It showed that 1928, 3219 and 2646 differentially expressed genes (DEGs) were identified among T3 vs. T0, T7 vs. T0, T15 vs. T0 respectively. Through Gene Ontology (GO) analyses of the DEGs from these comparisons, it revealed that ORFV provoked the vigorous immune response of the host cells during the early stage of infection. Moreover, GO and network analysis revealed that positive and negative regulative mechanisms of apoptosis worked as whole in the host cells, in order to reach a homeostasis of oral mucosa tissues.
Project description:Reverse genetics has been widely used to investigate function of viral genes. In the present study we investigated the gene expression profile of a primary ovine cell (OFTu) in response to infection with the wild type (OV-IA82) and deletion mutant virus (OV-IA82Δ024) aiming to determine possible functions for ORFV024 during ORFV infection. We used microarray analysis to investigate gene expression profile of OFTu with ORFV OV-IA82 and OV-IA82Δ024.
Project description:Using RNA sequencing technology, we compared specific changes in the transcriptomic profiles in human foreskin fibroblast cells (HFF-1) following ORFV infection. ORFV specifically upregulated and downregulated a variety of genes, including genes involved in antiviral immune response, apoptosis, cell cycle and a series of signaling pathways, such as the IFN and p53-signaling pathways. A range of genes were downregulated at early stage. In contrast, the virus up-regulates a number of cellular genes at late stage.
Project description:To study the oncolytic effect of ORFV on tumors, we performed ORFV treatment (i.t.) in B16 engrafted tumors, changes of gene transcripts were detected