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Brachyspira

ABSTRACT: Brachyspira species (mostly Brachyspira pilosicoli)sequencing

PROVIDER: PRJNA564276 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	VYIL01.dat.gz	Other
	VYIL01.fasta.gz	Fasta.gz
	VYIL01.master.dat	Other
	VYIM01.dat.gz	Other

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Project description:Applying a shotgun proteomics approach, we built a unique database of Brachyspira hyodysenteriae and B. pilosicoli peptides and proteins. A total of 148 LC-MS/MS runs were conducted on an Orbitrap instrument, and more than 29000 different peptides in cell supernatants and partially digested cell suspensions of 3 strains of B. hyodysenteriae and 2 strains of B. pilosicoli were identified. Overall, 16970 and 15493 peptide sequences, which pointed to 1625 and 1338 different protein accessions, were identified in B. hyodysenteriae and B. pilosicoli, respectively. The large-scale characterization of peptides and proteins in the extracellular medium as well as of the exposed proteins on the bacterial surface in these species provides a unique collection of interrelated data on the molecules involved in the interaction of these bacteria with their environment.

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BPP43_05035 is a Brachyspira pilosicoli cell surface adhesin that weakens the integrity of the epithelial barrier during infection

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