Transcriptional changes after YAP/TAZ siRNA-mediated knockdown, and BMP-2 stimulation and SMAD1 ChIP-Seq in BMP2-treated human fetal osteoblasts
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ABSTRACT:
PROVIDER: PRJNA564311 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR10080417_1.fastq.gz | Fastqsanger.gz | |||
| SRR10080417_2.fastq.gz | Fastqsanger.gz | |||
| SRR10080418_1.fastq.gz | Fastqsanger.gz | |||
| SRR10080418_2.fastq.gz | Fastqsanger.gz | |||
| SRR10080419_1.fastq.gz | Fastqsanger.gz |
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Project description:Osteogenesis is influenced by a variety of factors including growth factors - especially those of the TGF-beta superfamily - and Hippo signaling. Consequently, we hypothesize that the YAP/TAZ pathway is intertwining with TGF-beta and BMP signaling resulting in osteogenic differentiation. Therefore, we performed a comprehensive RNA-Seq study including both YAP/TAZ depletion by siRNA and BMP stimulation conditions in human fetal osteoblasts cell line hFOB 1.10. In order to identify the direct SMAD1 target genes corresponding to our RNA-Seq data, we performed an SMAD1 chromatin immunoprecipitation followed by sequencing.
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