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SMAD1/5 binding regions of human umbilical vein endothelial cells (HUVECs) treated with BMP.

ABSTRACT: Smad1/5 binding regions were identified by ChIP-seq. HUVECs were treated with BMP for 1.5 h and anti-SMAD1/5 ChIP-seq analyses were performed using Illumina genome analyzer.

ORGANISM(S): Homo sapiens

SUBMITTER: Daizo Koinuma

PROVIDER: E-GEOD-27634 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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ChIP-seq reveals cell type-specific binding patterns of BMP-specific Smads and a novel binding motif.

Morikawa Masato M Koinuma Daizo D Tsutsumi Shuichi S Vasilaki Eleftheria E Kanki Yasuharu Y Heldin Carl-Henrik CH Aburatani Hiroyuki H Miyazono Kohei K

Nucleic acids research 20110715 20

Dysregulated bone morphogenetic protein (BMP) signaling in endothelial cells (ECs) and pulmonary arterial smooth muscle cells (PASMCs) are implicated in human genetic disorders. Here, we generated genome-wide maps of Smad1/5 binding sites in ECs and PASMCs. Smad1/5 preferentially bound to the region outside the promoter of known genes, and the binding was associated with target gene upregulation. Cell-selective Smad1/5 binding patterns appear to be determined mostly by cell-specific differences ...[more]

PMID: 21764776

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