Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human bone marrow-derived mesenchymal stromal/stem cells retain their trophic repair and immunoregulatory properties but lose their multipotent differentiation capacity after extensive ageing in vitro

ABSTRACT: Human bone marrow-derived mesenchymal stromal/stem cells retain their trophic repair and immunoregulatory properties but lose their multipotent differentiation capacity after extensive ageing in vitro

PROVIDER: PRJNA564771 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10093216_1.fastq.gz	Fastqsanger.gz
	SRR10093216_2.fastq.gz	Fastqsanger.gz
	SRR10093217_1.fastq.gz	Fastqsanger.gz
	SRR10093217_2.fastq.gz	Fastqsanger.gz
	SRR10093218_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 36

Similar Datasets

Human bone marrow-derived mesenchymal stromal/stem cells retain their trophic repair and immunoregulatory properties but lose their multipotent differentiation capacity after extensive ageing in vitro

Project description:To assess the hierarchical importance of multipotent differentiation compared with trophic/immunoregulatory functions of MSCs, we have undertaken a detailed analysis of how these properties change with in vitro ageing of the cells. Using in vitro differentiation and repair models and integrating transcriptomics and proteomics data, we show that the multipotent differentiation capacity of MSCs falls with increasing passage, whereas trophic repair and immunoregulatory potency are maintained.

2020-05-15 | GSE137186 | GEO

Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factors

Project description:Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factors

| PRJNA887408 | ENA

Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factors

Project description:Signal transducer and activator of transcription 5 (STAT5a and STAT5b) are intrinsically critical for normal hematopoiesis but are also expressed in stromal cells. However, hematopoiesis-supporting stromal function has not been reported. Here, STAT5ab knockout (KO) was generated with a variety of bone marrow hematopoietic and stromal Cre transgenic mouse strains. Pan-hematopoietic deletion with Vav1-Cre was the positive control for loss of multipotent hematopoietic function but surprisingly dysregulated niche factor mRNA expression and deleted STAT5ab in CD45neg cells. Single cell transcriptome analysis of mouse bone marrow from wild-type or Vav1-Cre KO mice showed major changes in hematopoietic stem cell myeloid commitment genes and upregulated protein translation genes throughout myeloid-primed clusters.

2023-10-01 | GSE214857 | GEO

Megakaryocyte derived immunoregulatory cells regulate host-defense immunity against bacterial pathogens

Project description:Here, using single-cell sequencing we identified an MK derived immunoregulatory cell population, which plays an important role in host-protective responses against bacteria. In contrast to platelet-generating MKs, MK derived immunoregulatory cells highly express immune response genes and chemokines.

2022-03-24 | GSE168224 | GEO

Chromatin Signatures in Multipotent Human HSCs Indicate the Fate of Bivalent Genes during Differentiation

Project description:Histone modifications have been implicated in stem cell maintenance and differentiation. We have analyzed genome-wide changes in gene expression and histone modifications during differentiation of multipotent human primary hematopoietic stem cells/progenitor cells (HSCs/HPCs) into erythrocyte precursors. Our data indicate that H3K4me1, H3K9me1, and H3K27me1 associate with enhancers of differentiation genes prior to their activation and correlate with basal expression, suggesting that these monomethylations are involved in the maintenance of activation potential required for differentiation. In addition, although the majority of genes associated with both H3K4me3 and H3K27me3 in HSCs/HPCs become silent and lose H3K4me3 after differentiation, those that lose H3K27me3 and become activated after differentiation are associated with increased levels of H2A.Z, H3K4me1, H3K9me1, H4K20me1, and RNA polymerase II in HSCs/HPCs. Thus, our data suggest that gene expression changes during differentiation are programmed by chromatin modifications present at the HSC/HPC stage and provide a resource for enhancer and promoter identification. Genome-wide analysis of 8 histone methylations, H2AZ, and RNA Polymerase II in human CD133+ and CD36+ cells. (20 samples in total) and global change in gene expression in human CD133+ and CD36+ cells. (4 samples in total)

2009-01-09 | E-GEOD-12646 | biostudies-arrayexpress

Proteomes of Spirodela polyhriza under different trophic modes

Project description:The greater duckweed (Spirodela polyrhiza 7498) exhibits trophic diversity (photoautotrophic, heterotrophic, photoheterotrophic, and mixotrophic growth) depending on the availability of exogenous organic carbon sources and light. Here, we show that the ability to transition between various trophic growth conditions is an advantageous trait, providing great phenotypic plasticity and metabolic flexibility in S. polyrhiza 7498. By comparing S. polyrhiza 7498 growth characteristics, metabolic acclimation, and cellular ultrastructure across these trophic modes, we show that mixotrophy decreases photosynthetic performance and relieves the CO2 limitation of photosynthesis by enhancing the CO2 supply through the active respiration pathway. Proteomic and metabolomic analyses corroborated that S. polyrhiza 7498 increases its intracellular CO2 and decreases reactive oxygen species undermixotrophic and heterotrophic conditions, which substantially suppressed the wasteful photorespiration and oxidative-damage pathways. As a consequence, mixotrophy resulted in a higher biomass yield than the sum of photoautotrophy and heterotrophy.Our work provides a basis for using trophic transitions in S. polyrhiza 7498 for the enhanced accumulation of value-added products.

2023-02-27 | PXD040410 |

Pro-osteogenic trophic effects by PKA activation in human mesenchymal stromal cells

Project description:Human mesenchymal stromal cells (hMSCs) are able to differentiate into a wide variety of cell types, which makes them an interesting source for tissue engineering applications. On the other hand, these cells also secrete a broad panel of growth factors and cytokines that can exert trophic effects on surrounding tissues. In bone tissue engineering applications, the general assumption is that direct differentiation of hMSCs into osteoblasts accounts for newly observed bone formation in vivo. However, the secretion of bone-specific growth factors, but also pro-angiogenic factors, could also contribute to this process. We recently demonstrated that secretion of bone specific growth factors can be enhanced by treatment of hMSCs with the small molecule db-cAMP (cAMP) and here we investigate the biological activity of these secreted factors. We demonstrate that conditioned medium contains a variety of secreted growth factors, with differences between medium from basic-treated and cAMP-treated hMSCs. We show that conditioned medium from cAMP-treated hMSCs increases proliferation of various cell types and also induces osteogenic differentiation, whereas it has differential effects on migration. Microarray analysis on hMSCs exposed to conditioned medium confirmed upregulation of pathways involved in proliferation as well as osteogenic differentiation. Our data suggests that trophic factors secreted by hMSCs can be tuned for specific applications and that a good balance between differentiation on the one hand and secretion of bone trophic factors on the other, could potentially enhance bone formation for bone tissue engineering applications. For more information check: https://cbit.maastrichtuniversity.nl/

2018-10-06 | E-MTAB-7224 | biostudies-arrayexpress

Trophic State Proteomics of Chromochloris Time Course

Project description:Proteomic response of Chromochochloris WT and hxk1 strains to iron and glucose. WT-Fe+Glc cultures distinctly lose oxygenic photosynthetic capacity.

2023-06-13 | MSV000092155 | MassIVE

Chromatin Signatures in Multipotent Human HSCs Indicate the Fate of Bivalent Genes during Differentiation

2009-01-09 | GSE12646 | GEO

Connexin 43 controls the immunoregulatory phenotype of astrocytes

Project description:Connexin 43 controls the immunoregulatory phenotype of astrocytes

| PRJNA434418 | ENA