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The fibronectin mediated endothelial rescue effect in CCM3-deficient cells is independent from acute mRNA changes

ABSTRACT: The fibronectin mediated endothelial rescue effect in CCM3-deficient cells is independent from acute mRNA changes

PROVIDER: PRJNA565418 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10117833_1.fastq.gz	Fastqsanger.gz
	SRR10117833_2.fastq.gz	Fastqsanger.gz
	SRR10117834_1.fastq.gz	Fastqsanger.gz
	SRR10117834_2.fastq.gz	Fastqsanger.gz
	SRR10117835_1.fastq.gz	Fastqsanger.gz

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The fibronectin mediated endothelial rescue effect in CCM3-deficient cells is independent from acute mRNA changes

Project description:Using CRISPR/Cas9 genome editing and RNA sequencing, we demonstrate that fibronectin supplementation restores the impaired spheroid formation and attenuates actin stress fiber assembly in CCM3-/- endothelial cells independently of acute gene expression differences. In particular, there was no significant downregulation of well-known key players in CCM pathogenesis like KLF2 or KLF4.

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mRNA expression changes in CCM3-deficient endothelial cells

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CRISPR/Cas9 genome editing demonstrates that CCM3 modulates endothelial miRNA expression

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mRNA expression changes in CCM3-deficient endothelial cells

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Project description:The purpose of this study was to investigate the transcriptional regulation of endothelial cells in CCM pathology. We isolated cerebellar endothelial cells from healthy (wild-type) mice and from mice with an inducible endothelial-specific knock-out of the Ccm3 gene (Ccm3 knock-out). We prepared RNA libraries and sequenced the samples on a HiSeq2500 sequencing system with v4 chemistry from Illumina. We then performed a differential gene expression analysis to identify genes that play a role in CCM pathology.

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CCM3 is a gatekeeper in focal adhesions regulating mechanotransduction and YAP/TAZ signalling

Project description:We report that loss of CCM3/PDCD10 in fibroblasts induces FAK/Src-paxillin signalling driving actomyosin-dependent mechanotransduction leading to YAP/TAZ signalling. In vivo, loss of CCM3 in fibroblasts drives excessive tissue remodelling leading to the dissemination of breast cancer cells to distant organs.

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Fibronectin Induced Cardiac Myocyte Hypertrophy

Project description:Gene expression changes in neonatal rat ventricular myocytes plated in fibronectin (2 samples) or uncoated plates (2 samples). Keywords = Hypertrophy Cardiac Myocyte Fibronectin Keywords: repeat sample

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Early and late passage endothelial cells with or without PDCD10 downregulation

Project description:Primary cells deficient for PDCD10/CCM3 do not enter senescence as control cells. Microarray analysis was performed in cells transduced with non-targeting shRNA and CCM3 shRNA at passage 7 (early passage) and passage 11 (late passage), when control cells are already senescent. Primary endothelial cells were transduced either with non-target shRNA or with CCM3 shRNA. RNA was extracted at passage 7 and passage 11

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Effect of high or low chronic shear stress on microvascular endothelial cells cultured on native- versus AGE-fibronectin

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Effect of high or low chronic shear stress on microvascular endothelial cells cultured on native- versus AGE-fibronectin

2005-12-31 | E-GEOD-2424 | biostudies-arrayexpress