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Gene expression analysis of in vitro activated B cells from C57BL/6 and Ackr4-deficient mice

ABSTRACT: Gene expression analysis of in vitro activated B cells from C57BL/6 and Ackr4-deficient mice

PROVIDER: PRJNA575107 | ENA |

REPOSITORIES: ENA

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Gene expression analysis of in vitro activated B cells from C57BL/6 and Ackr4-deficient mice

Project description:To further understand differential phenotypes between C57BL/6 and Ackr4-deficient B cells, we have employed whole genome microarray expression profiling as a discovery platform to identify genes which are differentially expressed in the two genotypes. Murine B cells from spleens of C57BL/6 and Ackr4-deficient mice were purified by negative magnetic activated cell sorting (MACS) and activated in vitro with 10 µg/mL polyclonal anti-IgM and10 µg/mL anti-CD40 (clone FGK4.5) antibodies for three days in culture (96well plate, 37°C, 5%CO2). On day three, living B cells were sorted and RNA was extracted.

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RNAseq profiling of miR-132/212-deficient CD4+ T cells activated in vitro and in vivo.

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Expression data of primary melanocytes from aryl hydrocarbon deficient mice and corresponding wild-type C57BL/6 mice

Project description:[original Title] Comparison of expression data of primary murine melanocytes from aryl hydrocarbon deficient mice and corresponding wild-type C57BL/6 mice Melanin is produced exclusively by melanocytes and melanogenesis is the vital response to protect skin cells against Ultraviolet B (UVB)-induced DNA damage. The aryl hydrocarbon receptor (AhR) is a transcription factor, which may be involved in the physiological tanning response. Normal murine melanocytes express functional AhR. We tested gene expression in WT versus AhR-deficient mice primary murine melanocytes, isolated from the skin and cultivated for several passages. Skin epidermal cells from 2 individual C57BL/6 mice and 2 individual AhR-deficient mice (deletion of exon2, AhRtm1Bra) were grown for 6-8 weeks in selection medium to propagate melanocytes.

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Multi-omics Analysis of Salmonella enterica Serovar Typhimurium Infection of Wild Type C57BL/6 and Immunodeficient gp91-/-phox Mice

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Expression data of primary melanocytes from aryl hydrocarbon deficient mice and corresponding wild-type C57BL/6 mice

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C57BL/6 mice

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2011-12-29 | GSE30140 | GEO

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