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Transcriptome analysis of Botrytis cinerea in response to tea tree oil and its two characteristic components

ABSTRACT: Transcriptome analysis of Botrytis cinerea in response to tea tree oil and its two characteristic components

PROVIDER: PRJNA575349 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10215633_1.fastq.gz	Fastqsanger.gz
	SRR10215633_2.fastq.gz	Fastqsanger.gz
	SRR10215634_1.fastq.gz	Fastqsanger.gz
	SRR10215634_2.fastq.gz	Fastqsanger.gz
	SRR10215635_1.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of Botrytis cinerea in response to tea tree oil and its two characteristic components

Project description:Purpose: High-throughput RNA sequencing has been used to examine mRNA expression profiles in fungal cells treated with essential oils. The goals of this study are to analyze the global gene expression profiles in Botrytis cinerea with or without tea tree oil and its two characteristic components treatment by RNA-Seq. Methods: The mRNA profiles of Botrytis cinerea with or without tea tree oil and its two characteristic components treatment were generated by deep sequencing, in triplicate, using Illumina HiSeq™ 2500 sequencing platform. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR was performed to verified the sensitivity of the RNA-seq method. Results: After high-throughput RNA sequencing, reads were filtered to yield 111.22 Gb of clean sequence data. The GC content for all samples exceeded 45%. The Q20 ratio (used to evaluate reads quality) was greater than 94%, and Q30 base percentage was at least 87.07%. Altered expression of 7 genes was confirmed with qRT–PCR, demonstrating the high degree of sensitivity of the RNA-seq method. Most differentially expressed genes (DEGs) from B. cinera cells treated with terpinen-4-ol participated in biosynthesis of secondary metabolites, and the metabolism of amino acid, carbohydrate and lipid. 1,8-cineole mainly affected DEGs involved in genetic information processing, and thus inducing cell death. Conclusions: Terpinen-4-ol exerts antifungal activity mainly by blocking the expression of genes related to cell integrity and mitochondrial function. 1,8-cineole primarily affects genes involved in genetic information processing including DNA replication, transcription and repair. This study provides insight into the molecular mechanism by which tea tree oil acts against Botrytis cinerea based on the data from RNA-seq.

2019-10-03 | GSE138291 | GEO

Whole Genome Bisulfite Sequencing of Botrytis cinerea

Project description:Analysis of the genome-wide DNA methylation pattern of Botrytis cinerea. Results provide new and important information that DNA methylation is critical for pathogenicity and development of Botrytis cinerea by regulating gene expression.

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Whole Genome Bisulfite Sequencing Data of Botrytis cinerea

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PPH24 - Botrytis cinerea Steroidal glycoalkaloids

Project description:Multiple mechanisms of the grey mould Botrytis cinerea to detoxify plant antifungal steroidal glycoalkaloids

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Botrytis cinerea genes activated in infection process

Project description:To screen Botrytis genes activated in infection process, we performed gene expression profiling analysis using data obtained from RNA-seq of Botrytis cinerea cultured in vitro or infecting Arabidopsis leaves.

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WRKY33-dependent expression of Arabidopsis genes upon Botrytis cinerea 2100 inoculation

Project description:The Arabidopsis thaliana mutant wrky33 is highly susceptible to the necrotrophic fungus Botrytis cinerea. Comparing the expression profiles of B. cinerea-infected wrky33 and WT plants we identified 2765 differentially expressed genes dependent on WRKY33, of which 1675 were up-regulated in the mutant (termed WRKY33-repressed genes) and 1090 were down-regulated in the mutant. Combined with ChIP-seq data 318 genes were identified as direct functional targets of WRKY33 at 14 h post inoculation with spores of Botrytis cinerea 2100. Comparison of altered gene expression in Arabidopsis WT and wrky33 mutant plants 14 hours post inoculation with Botrytis cinerea 2100.

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Effect of depletion of NUP62 on gene expression during Botrytis cinerea infection of Arabidopsis seedlings [Arabidopsis]

Project description:To investigate NUP62 in the regulation of plant defense against Botrytis cinerea , we performed gene expression profiling analysis using data obtained from RNA-seq of nup62 mutant and WT arabidopsis with or without Botrytis cinerea infection.

2023-04-12 | GSE217020 | GEO

WRKY33-dependent expression of Arabidopsis genes upon Botrytis cinerea 2100 inoculation

2015-06-15 | GSE66290 | GEO

Next generation sequencing analysis of ginseng transcriptomes in response to Botrytis cinerea △BcSpd1 treatment

Project description:Next generation sequencing (NGS) was performed to identify genes changed in ginseng upon Botrytis cinerea △BcSpd1 treatment. The goal of the work is to find interesting genes involved in ginseng in response to fungi induction. The object is to reveal the molecular mechanism of ginseng defense induced by Botrytis cinerea △BcSpd1 .

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