Project description:This project is about the proteomic and phosphoproteomic analyses of phage JSS1 or JSS1Δ004 infected Salmonella strains. AD032TQ is proteomes of phage JSS1 or JSS1Δ004 infected S. enterica serovar Cerro 87. AD032TPST is phosphoproteome of phage JSS1 or JSS1Δ004 infected S. enterica serovar Cerro 87. AE065TQ is proteomes of phage JSS1 or JSS1Δ004 infected CX1, a S. enterica serovar Cerro 87 dndFGH deficient strain, harboring a plasmid expressing DndFGH from Escherichia coli B7A. AE065TPST is phosphoproteome of phage JSS1 or JSS1Δ004 infected CX1 harboring a plasmid expressing DndFGH from E. coli B7A.
Project description:This project is about the proteomic and phosphoproteomic analyses of phage JSS1 or JSS1Δ004 infected Salmonella strains. AD032TQ is proteomes of phage JSS1 or JSS1Δ004 infected S. enterica serovar Cerro 87. AD032TPST is phosphoproteome of phage JSS1 or JSS1Δ004 infected S. enterica serovar Cerro 87. AE065TQ is proteomes of phage JSS1 or JSS1Δ004 infected CX1, a S. enterica serovar Cerro 87 dndFGH deficient strain, harboring a plasmid expressing DndFGH from Escherichia coli B7A. AE065TPST is phosphoproteome of phage JSS1 or JSS1Δ004 infected CX1 harboring a plasmid expressing DndFGH from E. coli B7A.
Project description:This project is about the proteomic and phosphoproteomic analyses of phage JSS1 or JSS1Δ004 infected Salmonella strains. AD032TQ is proteomes of phage JSS1 or JSS1Δ004 infected S. enterica serovar Cerro 87. AD032TPST is phosphoproteome of phage JSS1 or JSS1Δ004 infected S. enterica serovar Cerro 87. AE065TQ is proteomes of phage JSS1 or JSS1Δ004 infected CX1, a S. enterica serovar Cerro 87 dndFGH deficient strain, harboring a plasmid expressing DndFGH from Escherichia coli B7A. AE065TPST is phosphoproteome of phage JSS1 or JSS1Δ004 infected CX1 harboring a plasmid expressing DndFGH from E. coli B7A.
Project description:This project is about the proteomic and phosphoproteomic analyses of phage JSS1 or JSS1Δ004 infected Salmonella strains. AD032TQ is proteomes of phage JSS1 or JSS1Δ004 infected S. enterica serovar Cerro 87. AD032TPST is phosphoproteome of phage JSS1 or JSS1Δ004 infected S. enterica serovar Cerro 87. AE065TQ is proteomes of phage JSS1 or JSS1Δ004 infected CX1, a S. enterica serovar Cerro 87 dndFGH deficient strain, harboring a plasmid expressing DndFGH from Escherichia coli B7A. AE065TPST is phosphoproteome of phage JSS1 or JSS1Δ004 infected CX1 harboring a plasmid expressing DndFGH from E. coli B7A.
Project description:Transcriptomic analysis in a Salmonella enterica Serovar Typhimurium SL 1344 that constitutively expresses stdE and stdF compared with a strain carrying an stdEF deletion A four chip study using total RNA recovered from two separate cultures of Salmonella enterica Serovar Typhimurium SL 1344 constitutively expressing stdE and stdF and two separate cultures of Salmonella enterica Serovar Typhimurium SL 1344 lacking stdE and stdF.
Project description:Transcriptomic analysis in a Salmonella enterica Serovar Typhimurium SL 1344 that constitutively expresses stdE and stdF compared with a strain carrying an stdEF deletion
Project description:Transcriptional profile of Salmonella enterica sv Typhimurium SL1344 ΔSTM2239 mutant cultures containing either a plasmid control (pFLAG-CTC) or a plasmid carrying STM2239 (pFLAG-STM2239) in the presence of IPTG.
Project description:The transcriptomes of Salmonella enterica serovar Typhimurium SL1344 and an isogenic ramA gene knockout mutant which contained ramA upon an IPTG-inducible over-expression plasmid.
Project description:Multidrug-resistant (MDR; resistance to >3 antimicrobial classes) Salmonella enterica serovar I 4,[5],12:i:- strains were linked to a 2015 foodborne outbreak from pork. Strain USDA15WA-1, associated with the outbreak, harbors an MDR module and the metal tolerance element Salmonella Genomic Island 4 (SGI-4). Characterization of SGI-4 revealed that conjugational transfer of SGI-4 resulted in the mobile genetic element (MGE) replicating as a plasmid or integrating into the chromosome. Tolerance to copper, arsenic, and antimony compounds was increased in Salmonella strains containing SGI-4 compared to strains lacking the MGE. Following Salmonella exposure to copper, RNA-seq transcriptional analysis demonstrated significant differential expression of diverse genes and pathways, including induction of numerous metal tolerance genes (copper, arsenic, silver, and mercury). Evaluation of swine administered elevated concentrations of zinc oxide (2,000 mg/kg) and copper sulfate (200 mg/kg) as an antimicrobial feed additive (Zn+Cu) in their diet for 4 weeks prior to and 3 weeks post-inoculation with serovar I 4,[5],12:i:- indicated that Salmonella shedding levels declined at a slower rate in pigs receiving in-feed Zn+Cu compared to control pigs (no Zn+Cu). The presence of metal tolerance genes in MDR Salmonella serovar I 4,[5],12:i:- may provide benefits for environmental survival or swine colonization in metal-containing settings.
Project description:Investigation of whole genome gene expression level changes in a Salmonella enterica serovar Typhimurium UK1 delta-iacP mutant, compared to the wild-type strain. IacP is resoponsible for the secretion of virulence effector proteins via the type III secretion system, thereby contributing the virulence of S. Typhimurium. The mutants analyzed in this study are further described in Kim et al. 2011. Role of Salmonella Pathogenicity Island 1 Protein IacP in Salmonella enterica Serovar Typhimurium Pathogenesis. Infection and Immunity 79(4):1440-1450 (PMID 21263021). A chip study using total RNA recovered from two separate wild-type cultures of Salmonella enterica serovar Typhimurium UK1 and two separate cultures of a mutant strain, Salmonella enterica serovar Typhimurium UK1 delta-iacP. Each chip measures the expression level of 4,302 genes from Salmonella enterica serovar Typhimurium.