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Transcription profiling of Salmonella enterica serovar Typhimurium SL1344 and a ramA over-expressing strain

ABSTRACT: The transcriptomes of Salmonella enterica serovar Typhimurium SL1344 and an isogenic ramA gene knockout mutant which contained ramA upon an IPTG-inducible over-expression plasmid.

ORGANISM(S): Salmonella typhimurium

SUBMITTER: Andrew Bailey

PROVIDER: E-MEXP-2195 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RamA, a member of the AraC/XylS family, influences both virulence and efflux in Salmonella enterica serovar Typhimurium.

Bailey Andrew M AM Ivens Al A Kingsley Rob R Cottell Jennifer L JL Wain John J Piddock Laura J V LJ

Journal of bacteriology 20100115 6

The transcriptomes of Salmonella enterica serovar Typhimurium SL1344 lacking a functional ramA or ramR or with plasmid-mediated high-level overexpression of ramA were compared to those of the wild-type parental strain. Inactivation of ramA led to increased expression of 14 SPI-1 genes and decreased expression of three SPI-2 genes, and it altered expression of ribosomal biosynthetic genes and several amino acid biosynthetic pathways. Furthermore, disruption of ramA led to decreased survival withi ...[more]

PMID: 20081028

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Transcription profiling of Salmonella enterica serovar Typhimurium SL1344 and a SL1344 ramA gene knockout

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Minimal vs LB media comparison

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