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ABSTRACT: Identification of microRNAs from the stems of three Dendrobium species

PROVIDER: PRJNA576944 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10259377.fastq.gz	Fastqsanger.gz
	SRR10259378.fastq.gz	Fastqsanger.gz
	SRR10259379.fastq.gz	Fastqsanger.gz
	SRR10259380.fastq.gz	Fastqsanger.gz
	SRR10259381.fastq.gz	Fastqsanger.gz

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Project description:Identification of microRNA targets from the stems of three Dendrobium species--degradome-seq

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Illumina sequencing reveals conserved and novel microRNAs involved in dendrobine biosynthesis in Dendrobium nobile

Project description:Dendrobium plants are perennial herbs in the family Orchidaceae (Dendrobium Sw.). Due to protocorm can also produce plant-specific useful metabolites, protocorm is becoming a good substitute. MicroRNAs play essential roles in plant growth, development, and the response to environmental stresses, and they are widely used for prediction of molecular functions for biosynthesizing active comportments in medicinal plants. To obtain insight into the function of miRNAs in Dendrobium plants. Illumina sequencing of D. nobile protocorm, D. officinale protocorm and D. nobile leaf were conducted. A total of 439, 412 and 432 miRNAs were identified in three samples, and their expression levels were significantly different. Specially, 2, 12 and 4 specific miRNAs were identified. Through integrated GO and KEGG function annotation, miRNAs mainly involved in metabolic pathways, plant hormone signal transduction, biological regulation and protein binding. AACT, MK, DXR and HDS as important enzymes in synthesizing basic precursor isoprene pyrophosphate (IPP). were predicted controlled by 6 different miRNAs in terpenoid backbone biosynthesis pathway. 26 miRNAs participated in Auxin, Cytoklinine, Abscisic acid, Jasmonic acid and Salicylic acid signal transduction pathway. Our results could provide valuable information about miRNAs involved in terpenoid biosynthesis and plant hormone signal transduction pathway in D. nobile and candidate genes for increasing the yield of dendrobine.

2022-08-01 | GSE135444 | GEO

Project description:Identification of three novel species

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microRNAs in Ichthyosporea

Project description:Identification of microRNAs in ichthyosporean species

| PRJEB51319 | ENA

Project description:Drought stress induced different response mechanisms in three Dendrobium species

| PRJNA1055284 | ENA

Project description:Endophytic bacteria in dendrobium stems of different lengths

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Clinical molecular subtyping study based on precision Fusobacterium detection in colrectal cancer.

Project description:Interventions: CRC group:Nil;Healthy control group:Nil Primary outcome(s): Identification of clostridium species Study Design: Factorial

| 2697414 | ecrin-mdr-crc

Protein solution method for direct identification of Arabidopsis stem proteins

Project description:Identifying the intracellular and cell wall-ionically bound glycoside hydrolases (GHs) carbohydrate esterases (CEs) at the late growth stage of Arabidopsis stems is important for understanding the mechanisms regulating the cell wall integrity. The fact that whole plant stems are used as biomass feedstocks and the mixing of intracellular proteins with the cell wall proteome caused by an increasing proportion of broken cells of stems at the late growth stage pose a challenge in identifying these GHs and CEs. Here, we used a CaCl2-extraction procedure to isolate non-structural proteins from Arabidopsis whole stems, followed by protein solution method for direct identification of stem proteins, and by SDS-PAGE separation and protein gel band method for improving the identification of stem proteins, using Nano-LC-MS/MS analysis. Totally, 75 and 236 stem proteins were identified by using these two methods, respectively, confirming the later method (i.e. protein gel method) identified three time more stem proteins. Among these proteins, based on cell wall protein databases and data mining analyses, 6 and 22 proteins were identified as cell wall proteins from the late growth stage Arabidopsis stems, by using these two methods respectively.

2015-05-11 | PXD001851 | Pride

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