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A bifunctional nucleosome binding protein mediates specialized mSWI/SNF complex targeting and activity (ChIP-seq dataset)

ABSTRACT: A bifunctional nucleosome binding protein mediates specialized mSWI/SNF complex targeting and activity (ChIP-seq dataset)

PROVIDER: PRJNA578157 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10303014.fastq.gz	Fastqsanger.gz
	SRR10303015.fastq.gz	Fastqsanger.gz
	SRR10303016.fastq.gz	Fastqsanger.gz
	SRR10303017.fastq.gz	Fastqsanger.gz
	SRR10303018.fastq.gz	Fastqsanger.gz

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A bifunctional nucleosome binding protein mediates specialized mSWI/SNF complex targeting and activity (ChIP-seq dataset)

Project description:Here we identify and characterize a minimal domain within the SS18-SSX fusion oncoprotein hallmark to synovial sarcoma, that exhibits a high-affinity interaction with the nucleosome acidic patch. We find that this region is necessary and sufficient for nucleosome binding, mSWI/SNF (BAF) chromatin remodeling complex targeting, catalytic activity, and activation of cancer-specific gene expression. Moreover, we identify PRC1-mediated H2AUb119 as a further rheostat governing further stability and binding. Together, our results define an unexpected direct bifunctional histone detector on SSX that is responsible for disease-specific chromatin recruitment of a major family of chromatin regulatory complexes.

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A bifunctional nucleosome binding protein mediates specialized mSWI/SNF complex targeting and activity

Project description:A bifunctional nucleosome binding protein mediates specialized mSWI/SNF complex targeting and activity

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A bifunctional nucleosome binding protein mediates specialized mSWI/SNF complex targeting and activity (RNA-seq dataset)

Project description:A bifunctional nucleosome binding protein mediates specialized mSWI/SNF complex targeting and activity (RNA-seq dataset)

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A bifunctional nucleosome binding protein mediates specialized mSWI/SNF complex targeting and activity

Project description:This SuperSeries is composed of the SubSeries listed below.

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Identifying the genomic regions co-bound by ASCL1 and mSWI/SNF remodelers during neural differentiation [ChIP-seq]

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Project description:Chromatin remodeling complexes regulate gene expression by shifting, evicting, and exchanging nucleosomes along the chromosomes of eukaryotic organisms. The mammalian SWI/SNF chromatin remodeling complex (mSWI/SNF or BAF) is mutated in over 20% of human cancers and loss of the SMARCB1 gene, encoding the BAF47 protein subunit, results in one of the most aggressive and lethal pediatric cancers. An accumulation of point mutations occurs at the C-terminal end of the protein, for which the functional ramifications are unknown. We previously demonstrated that reintroduction of SMARCB1 in SMARCB1-null malignant rhabdoid tumor cells results in a genome-wide increase of mSWI/SNF complex occupancy coupled with activation of PRC2-repressed genes. Here, we study the functional consequences that these point mutations exert on mSWI/SNF complex activity in SMARCB1-deficient tumor cells and extend this investigation to a CRISPR/Cas9-mediated SMARCB1-heterozygous mutant induced pluripotent stem cell. Intriguingly, we observe that the mutant complexes bind similarly to wild-type SMARCB1 complexes at enhancers throughout the genome but often fail to transcriptionally activate nearby genes in a cis-regulatory manner.

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