Project description:Heart failure (HF) after myocardial infarction (MI) is a serious health issue. This study investigates the therapeutic effects of Shen-Yuan-Dan Capsule (SYD) on post-MI HF and explores its mechanisms, particularly involving m6A modification and autophagy. Using network pharmacology and MeRIP-seq, we identified potential epitranscriptomic targets. In vitro, H9c2 cardiomyocytes were treated with Phenylephrine (PE) and SYD. MeRIP-seq analysis identified differential m6A peak distribution among control, PE-induced, and SYD-treated groups, revealing that SYD alleviates HF by regulating the mTOR/TFEB autophagy pathway through inhibition of METTL3-mediated m6A modification.
2026-03-02 | GSE320263 | GEO
Project description:Small RNA-seq and o8G-miSeq of Hs683 cell line
| PRJNA794930 | ENA
Project description:Small RNA-seq and o8G-miSeq of Huh7 cell line
| PRJNA794950 | ENA
Project description:Small RNA-seq and o8G-miSeq of HepG2 cell line
Project description:Background: Cellular senescence plays a key role in the development of cancer, but the underlying mechanisms are unknown.Recently, several recent studies have shown that RNA methylation is closely related to cancer cell aging. 8-Oxoguanine (o8G) is an important and widely distributed methylation modification whose role in cancer cell senescence is far from elucidated. Methods: In this study, senescent cancer cell models (CaCO2 cells) were constructed by knocking down the ADAR1 gene. RNA immunoprecipitation sequencing was used to identify the o 8 G peaks on messenger RNA (mRNA) of normal CaCO2 cells and senescent CaCO2 cells, and the distribution characteristics of mRNA o 8 G modification were identified. Further bioinformatics analysis of the sequencing data was performed to preliminarily elucidate the potential function of the o8G-modified mRNA. Results: There were significant differences in mRNA o 8 G modification distribution between normal and senescent CaCO2 cells.It is suggested that o8G modification may play a key role in inducing cancer cells or promoting cancer cell senescence. Gene ontology (GO) enrichment analysis showed that the genes modified by o 8 G were enriched in Cellular component organization or biogenesis, Focal adhesion,and RNA binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the genes modified by o8G areconcentrated in Focal adhesion signaling pathway, Small cell lung cancer signaling pathway and Proteoglycans in cancer signaling pathway. Conclusion: This study preliminarily revealed the different distribution patterns of o8G modification between normal CaCO2 cells and senescent CaCO2 cells. Our study established the link between o 8 G modification and cancer cell senescence, which provides a new insight into the mechanism of cancer cell senescence and a potential therapeutic target for subsequent cancer treatment.
2024-12-21 | GSE284992 | GEO
Project description:Small RNA-seq and o8G-miSeq of rat treated/untreated with DEN