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ABSTRACT: Small RNA-seq and o8G-miSeq of Huh7 cell line

PROVIDER: PRJNA794950 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17459578_1.fastq.gz	Fastqsanger.gz
	SRR17459578_2.fastq.gz	Fastqsanger.gz
	SRR17459579_1.fastq.gz	Fastqsanger.gz
	SRR17459579_2.fastq.gz	Fastqsanger.gz
	SRR17459580_1.fastq.gz	Fastqsanger.gz

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Host Factors with Reduced Expression in Two HCV Permissive Cell Lines as Compared to the Non-Permissive Parent Cell Line Huh7

Project description:Drugs directly targeting Hepatitis C (HCV) are often rendered useless by the high mutation rate of the virus. Thus, we deduce that targeting of host factor that affect HCV replication may provide enhanced therapy fort HCV infection. Hepatocyte cell line Huh7 is known to be non-permissive for Hepatits C (HCV) replication. Through a method developed by the Rice laboratory (Blight, K.J., et al., J Virol, 2002), selection of a small subset of permissive hepatocytes is possible. The Rice laboratory generated the first permissive cell line, Huh7.5, using this method. We generated another permissive cell line, HRP1, using the same method. With microarray, we compared the expression of host mRNAs in non-permissive Huh7 to both Huh7.5 and HRP1 searching for host factors lost in the cell lines permisive for HCV replication. Non-permissive cell line Huh7 and permissive cell lines Huh7.5 and HRP1 were harvested for RNA extraction and hybridization on Affymetrix microarrays.

2011-05-01 | E-GEOD-25156 | biostudies-arrayexpress

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