Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bulk RNA-sequencing of spleenic LCMV-specific memory TFH cells treated with aICOSL.

ABSTRACT: Bulk RNA-sequencing of spleenic LCMV-specific memory TFH cells treated with aICOSL.

PROVIDER: PRJNA578728 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10319963_1.fastq.gz	Fastqsanger.gz
	SRR10319963_2.fastq.gz	Fastqsanger.gz
	SRR10319964_1.fastq.gz	Fastqsanger.gz
	SRR10319964_2.fastq.gz	Fastqsanger.gz
	SRR10319965_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Bulk RNA-sequencing of spleenic LCMV-specific memory TFH cells treated with aICOSL.

Project description:We performed RNA-seq analysis of splenic samples from 8 individual mice sorted for LCMV-specific memory follicular B helper T cells (TFH) with or without aICOSL treatment (4 samples per group).

2019-12-05 | GSE139197 | GEO

Expression data from virus-specific mouse Th1 and Tfh CD4 T cell subsets after LCMV infection

Project description:CD4 T follicular helper (Tfh) cells provide the required signals to B cells for germinal center reactions that are necessary for longlived antibody responses. However, it remains unclear whether there are CD4+ memory T cells committed to the Tfh lineage after antigen clearance. Using adoptive transfer of antigen-specific memory CD4+ subpopulations (based on CXCR5 and Ly6c expression)in the LCMV infection model, we found that there are distinct memory CD4+ T cell populations with commitment to the Tfh and Th1 lineages. Our conclusions are based on gene expression profiles, epigenetic studies and phenotypic and functional analysis. The gene expression profiles of virus-specific CD4 T cell subets at effector and memory stages is presented here. The SMARTA TCR transgenic / adptive transfer system was used to identify and sort subsets of antigen-specific CD4 T cells (based on their expression of Ly6c and CXCR5) elicited after acute infection with LCMV (Arm).

2013-04-22 | E-GEOD-43863 | biostudies-arrayexpress

Expression data from virus-specific mouse Th1 and Tfh CD4 T cell subsets after LCMV infection

2013-04-22 | GSE43863 | GEO

Gene expression data from memory Rictor-/- and WT Tfh and Th1 cells in LCMV acute infection

Project description:Microarray experiments were performed to demonstrate the transcriptome changes due to Rictor deficiency in memory follicular T helper (Tfh) and Type 1 T helper (Th1) cells. These data revealed that the immunological memory formation and persistance associated genes are changed in Rictor-null memory Tfh and Th1 cells, indicating that Rictor is essential for memory CD4 T cell pool.

2021-06-06 | GSE151800 | GEO

Gene expression data from LCMV-specific WT and Tcf7-/- follicular T helper cells

Project description:We did microarray experiments to elucidate the transcriptome changes due to TCF-1 deficiency in Tfh cells. These data clearly demonstrate that Tfh-associated genes were down-regulated in TCF-1-null Tfh cells, whereas Th1-associated signatures were up-regulated, indicating that TCF-1 initiates the Tfh fate by directly strengthening Tfh signatures while suppressing Th1-associated genes. We first sorted WT and TCF-1-null Tfh cells (2 samples per group, each sample was pooled from 4 mice) at day 8 after LCMV Armstrong infection. Then, we extracted RNA, which were subsequently sent to microarray analysis.

2015-07-24 | E-GEOD-65693 | biostudies-arrayexpress

RNA-seq of memory CD4+ Helper T cell subsets (Th1 and Tfh) during LCMV-Armstrong infection

Project description:CD4+ memory T cells play a pivatol role in mediating long-term immunity and therefore are an important target in vaccine development. Multiple functionally distinct helper CD4+ T cell subsets can arise in response to a single invading pathogen, complicating the identification of rare memory CD4+ T cells. Here we found that expression of Id3, an inhibitor of E protein transcription factors, identified a population of cells within both the CD4+ Tfh and Th1 helper lineages that exhibited memory potential in response to secondary infection. Notably, a subset of Th1 memory cells expressing Id3 exhibited enhanced expansion upon response to pathogen, generating both Th1 and Tfh secondary effector cell populations, and displayed enrichment of key molecules associated with memory potential when compared to Id3lo Th1 cells. Thus, Id3 expression serves as an important marker of multipotent memory CD4+ T cells.

2022-07-01 | GSE175740 | GEO

Gene expression in WT and Thpok-deficient LCMV-specific memory T cells

Project description:Gene expression of memory CD4+ and CD8+ T cells determined by RNAseq 30 days after LCMV Armstrong infection

2019-01-08 | GSE116519 | GEO

Human Tfh cell RNA bulk sequencing

Project description:We RNA bulk sequenced human Tfh cells sorted from healthy control and MS patient CSF.

2019-12-11 | GSE141797 | GEO

RNA-seq of memory CD4+ Helper T cell subsets (Th1 and Tfh) during LCMV-Armstrong infection

Project description:RNA-seq of memory CD4+ Helper T cell subsets (Th1 and Tfh) during LCMV-Armstrong infection

| PRJNA733469 | ENA

Gene expression data from memory Rictor-/- and WT Tfh and Th1 cells in LCMV acute infection

Project description:Gene expression data from memory Rictor-/- and WT Tfh and Th1 cells in LCMV acute infection