Project description:This project was based on Tandem Mass Tag (TMT) and Parallel Reaction Monitoring (PRM) technologies to study the changes in protein expression of leaves in Sophora alopecuroides under 150 mM NaCl condition treated for 3 days and 7 days. It was worthy of attention that the expression of several transporters related to the second messenger signaling pathway, such as the phosphatidylinositol signaling system, cAMP signaling pathway, and calcium signaling pathway had changed when treated for 3 d. However, the expression of some transferase, oxidoreductase, dehydrogenase was involved in the biosynthesis of flavonoids, alkaloids, phenylpropanoids and certain amino acid metabolisms had changed when treated for 7 d. This study analyzed the proteomic profiling of Sophora alopecuroides in response to salt stress using high-throughput omics technology firstly. Many potential genes were discovered that might be involved in salt stress, which laid the foundation for further research on their functions. All the results are of great significance for the researchers who are focus on the molecular genetic machnisms of abiotic stresses in plants, especially under salt stress.
Project description:Three new isoflavone glucosides, kudonol A-C (1-3), two new ester derivatives of phenylpropanoid, kudolignan A and B (4-5) and five known compounds, (-)-maackiain (6), neoliquiritin (7), methyl 4-coumarate (8), methyl ferulate (9) and (+)-wikstromol (10), were isolated from an extract of dried seeds of the traditional Chinese medicinal plant Sophora alopecuroides L. Their structures were established by NMR and HRESIMS data analyses. The monosaccharide part's configuration of isoflavone glucosides was confirmed by acid hydrolysis and analyzed by a JAsco OR-4090 chiral detector, comparing it to standard substance D-glucose. The cytotoxicity effects against HeLa, Hep3B, MCF-7 and H1299 cells were tested by CCK-8 assay.
