Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Analysis of Circulating Breast Cancer Cell Heterogeneity and Interactions with Peripheral Blood Mononuclear Cells.

ABSTRACT: Analysis of Circulating Breast Cancer Cell Heterogeneity and Interactions with Peripheral Blood Mononuclear Cells.

PROVIDER: PRJNA579983 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Analysis of Circulating Breast Cancer Cell Heterogeneity and Interactions with Peripheral Blood Mononuclear Cells.

Project description:Purpose: To infer interactions between circulating breast cancer cells and peripheral mononuclear cells using bioinformatics analysis of single cell RNA-sequencing. Methods: Filter based method for capturing single cells from 7.5mL of blood from patients with breast cancer, followed by single cell RNA-sequencing. Results: Transcriptomes predicted for two CTC populations with distinct expression profiles and interactions with peripheral blood mononuclear cells.

2020-08-25 | GSE139495 | GEO

Analysis of circulating breast cancer cell heterogeneity and interactions with peripheral blood mononuclear cells.

Project description:For solid tumors, extravasation of cancer cells and their survival in circulation represents a critical stage of the metastatic process that lacks complete understanding. Gaining insight into interactions between circulating tumor cells (CTCs) and other peripheral blood mononuclear cells (PBMCs) may provide valuable prognostic information. The purpose of this study was to use single-cell RNA-sequencing (scRNA-seq) of liquid biopsies from breast cancer patients to begin defining intravascular interactions. We captured CTCs from the peripheral blood of breast cancer patients using size-exclusion membranes followed by scRNA-seq of enriched CTCs and carry-over PBMCs. Transcriptome analysis identified two populations of CTCs: one enriched for transcripts indicative of estrogen responsiveness and increased proliferation and another enriched for transcripts characteristic of reduced proliferation and epithelial-mesenchymal transition (EMT). We applied interactome and pathway analysis to determine interactions between CTCs and other captured cells. Our analysis predicted for enhanced immune evasion in the CTC population with EMT characteristics. In addition, PD-1/PD-L1 pathway activation and T cell exhaustion were predicted in T cells isolated from breast cancer patients compared with normal T cells. We conclude that scRNA-seq of breast cancer CTCs generally stratifies them into two types based on their proliferative and epithelial state and differential potential to interact with PBMCs. Better understanding of CTC subtypes and their intravascular interactions may help design treatments directed against CTCs with high metastatic and immune-evasive competence.

| S-EPMC7895311 | biostudies-literature

Microarray profiling peripheral blood mononuclear cells

Project description:Microarray profiling peripheral blood mononuclear cells

| PRJNA418401 | ENA

Proteome signatures of inflammatory activated primary human peripheral blood mononuclear cells

Project description:Primary human peripheral blood mononuclear cells were isolated from full blood by standard ficoll centrifugation. Cells were washed and processed immediately.

2012-07-16 | PRD000731 | Pride

Transcriptomes analysis of peripheral blood mononuclear cell revealed the immune heterogeneity of bTB at different stages

Project description:Transcriptomes analysis of peripheral blood mononuclear cell revealed the immune heterogeneity of bTB at different stages

| PRJNA628939 | ENA

Transcriptomes analysis of peripheral blood mononuclear cell revealed the immune heterogeneity of bTB at different stages

Project description:We investigated peripheral blood mononuclear cell (PBMC) transcriptomes in naturally M. bovis-infected cattle. PBMCs isolated from cattle with different infection statuses (i.e., nested PCR-positive (bTB PCR-P), nested PCR-negative (bTB PCR-N) M. bovis-infected cattle and healthy cattle) were treated with bovine purified protein derivative of bovine tuberculin (PPD-B) or phosphate-buffered saline (PBS) for 6 h. By RNA-Seq, we compared the transcriptomes of PPD-B-stimulated and -unstimulated (PBS-treated) PBMCs between the three groups. Numerous differentially expressed genes were identified following pair-wise comparison of different groups, with or without PPD-B stimulation (adjusted p < 0.05). RNA-seq data was confirmed by validating 14 known genes with qRT–PCR. Finally, data analysis revealed the immune heterogeneity of bTB at different stages which can improve the understanding of the molecular mechanisms in bTB progression.

2020-04-28 | GSE149505 | GEO

Proteomic characterization of peripheral blood mononuclear cells from ankylosing spondylitis patients

Project description:Ankylosing spondylitis (AS) is an inflammatory arthritis of the axial skeleton that predominantly affects young men. HLA-B27 has remained the major genetic risk factor in AS. Recently, more non-MHC genes has been discoverd to be involved in AS pathogenesis, especially IL-23 signalling pathway related genes. We performed a proteomic study of the peripheral blood mononuclear cells from AS patients and healthy donors.

2023-04-15 | PXD024989 | Pride

Transcriptome profiling of peripheral blood mononuclear cell subsets

Project description:Transcriptome profiling was conducted on 2 replicate samples of total peripheral blood mononuclear cells (PBMC) and isolated PBMC subsets.

2020-04-14 | GSE101489 | GEO

Immunophenotyping peripheral blood mononuclear cells in human HFpEF

Project description:Immunophenotyping peripheral blood mononuclear cells in human HFpEF

| PRJNA926645 | ENA

miR from peripheral blood mononuclear cells' (PBMCs') exosome

Project description:miR from peripheral blood mononuclear cells' (PBMCs') exosome

| PRJNA786954 | ENA